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Status
Public on Jan 31, 2005
Title
University of Goettingen TAL UniGene_Set_RZPD2_mm2 pattern 06102003
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Mus musculus
Description
Preparation of high-density cDNA microarrays. PCR-based cDNA amplification (Hedge et al., 2000 PMID: 10997270) was used to generate DNA from a murine cDNA clone library (Mouse Unigene Set RZPD 2) purchased from the RZPD (http://www.rzpd.de ). This murine library contained 21,826 non-redundant clones from diverse tissues. After fully automated PCR-amplification and ultrafiltration-based purification using a Beckman-Coulter cDNA-production unit, control gel electrophoresis was perfomed showing that 95% of the products were clonally amplified. Non clonal or failed PCR-products were excluded from data analysis. The PCR-products were spotted on epoxy-coated glass slides (Schott Nexterion) using the BioRad spotter "Virtek Chip-Writer Pro" with 48 split pins (purchased from Telechem, www.arrayit.com). The arrays were prepared for hybridisation using surface blocking as described by the slide manufacturer.
Web link
http://www.microarrays.med.uni-goettingen.de Contributor(s)
Spielbauer B , Landgrebe J Submission date
Jan 20, 2005
Last update date
Jan 17, 2013
Contact name
Bettina Spielbauer
E-mail(s)
bspielb@gwdg.de Phone
+49 (0) 551 39 5921
Organization name
University of Goettingen
Department
Neuro and Sensory Physiology
Street address
Humboldtallee 23
City
Göttingen
ZIP/Postal code
37073
Country
Germany
Samples (6)
GSM45178 , GSM45179 , GSM45180 , GSM45181 , GSM45182 , GSM45183 Series (1)
GSE2400 Amyotrophic lateral sclerosis: SOD1 mouse model
Data table header descriptions ID Spot ID, unique identifier
RZPD_ID Visit http://www.rzpd.de
CLONE_ID_ALL Visit http://www.rzpd.de; number = IMAGE clone, MMnumber = LION Bioscience´s clone
CLONE_ID SPOT_ID UNIGENE UniGene cluster ID, mus musculus build 129
GENE_SYMBOL Gene symbol, from UniGene build 129
GENE_NAME Descriptive gene name, from UniGene build 129
Data table ID RZPD_ID CLONE_ID_ALL CLONE_ID SPOT_ID UNIGENE GENE_SYMBOL GENE_NAME 386
RZPDp981A021D
778314
IMAGE:778314
336316
Ptprr
protein tyrosine phosphatase, receptor type, R
387
RZPDp981A031D
778062
IMAGE:778062
25988
Bcl2l10
Bcl2-like 10
388
RZPDp981A041D
777914
IMAGE:777914
296127
D430004I08Rik
RIKEN cDNA D430004I08 gene
389
RZPDp981A051D
778167
IMAGE:778167
1160
Transcribed sequence with weak similarity to protein pir:A45973 (H.sapiens) A45973 trichohyalin - human
390
RZPDp981A061D
777977
IMAGE:777977
270881
Transcribed sequences
391
RZPDp981A071D
777968
IMAGE:777968
291524
Similar to erythrocyte membrane protein 3
392
RZPDp981A081D
777994
IMAGE:777994
253819
Pik3r3
phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55)
393
RZPDp981A091D
836100
IMAGE:836100
37523
Transcribed sequences
394
RZPDp981A101D
778500
IMAGE:778500
395
RZPDp981A111D
777997
IMAGE:777997
3810
Pik3c2a
phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide
396
RZPDp981A121D
833250
IMAGE:833250
12831
3830421F13Rik
RIKEN cDNA 3830421F13 gene
397
RZPDp981A013D
1107993
IMAGE:1107993
27661
Mus musculus 0 day neonate head cDNA, RIKEN full-length enriched library, clone:4831440M03 product:unknown EST, full insert sequence
398
RZPDp981A023D
1108593
IMAGE:1108593
41035
Similar to Munc13-3
399
RZPDp981A033D
961705
IMAGE:961705
294313
Similar to hypothetical protein FLJ36180
400
RZPDp981A043D
1107286
IMAGE:1107286
211709
Mus musculus adult male liver tumor cDNA, RIKEN full-length enriched library, clone:C730045M19 product:unknown EST, full insert sequence
401
RZPDp981A053D
1107321
IMAGE:1107321
218166
Transcribed sequences
402
RZPDp981A063D
1125019
IMAGE:1125019
155595
Zfp371
zinc finger protein 371
403
RZPDp981A073D
1107531
IMAGE:1107531
134277
Mus musculus adult retina cDNA, RIKEN full-length enriched library, clone:A930027P06 product:unknown EST, full insert sequence
404
RZPDp981A083D
1124541
IMAGE:1124541
38884
3830408G10Rik
RIKEN cDNA 3830408G10 gene
405
RZPDp981A093D
1107870
IMAGE:1107870
319543
Transcribed sequence with weak similarity to protein sp:Q99676 (H.sapiens) Z184_HUMAN Zinc finger protein 184
20636 1900 Kbytes .Supplementary data files not provided
