hSOD1(G93A) transgenic mice: transgenic mice expressing the human SOD1 gene with a G93A mutation (Gurney et al., 1994 [PMID: 8209258]), purchased from The Jackson Laboratory (B6SJL-Tg(SOD1-G93A)1Gur/J, stock no. 002726 [http://www.jax.org/]); fALS (familial amyotrophic lateral sclerosis) mouse model. Control mice: non-transgenic littermates. Timepoint: 17 week-old mice; transgenic mice showed severe signs of paralysis. RNA preparation: isolation of total RNA from spinal cords using RNeasy Maxi Kit (Qiagen) followed by DNase I digestion. RNA processing: RNA amplification (BD Atlas SMART Fluorescent Probe Amplification Kit, starting material: 1000 ng of total RNA, optimised amplification for each sample, stopped at PCR-cycle 18, 19 or 20 within the exponential amplification phase), labeling of amplified cDNA with cy3-/cy5-dCTP (Qiagen LabelStar Array Kit, per array and dye, 1000 ng of amplified cDNA were labeled using two labeling-reactions from the kit). Microarray hybridisation and washing: according to the slide manufacturer´s recommendations (cf. GLP1800); microarray scanning: GMS 428 Microarray Scanner (AffyMetrix). Microarray raw data: software package AIM (Katzer et al., 2003 [PMID: 15376910]). Signals with low quality were excluded from data analysis. Normalisation: pin-wise lowess-regression based normalisation (Yang et al., 2002 [PMID: 11842121]). Keywords = amyotrophic lateral sclerosis, ALS, SOD1 mouse model
Normalised log2-ratio(Cy3/Cy5) (ratio: background corrected means of signal intensities). (Datasets with empty 'VALUES' were excluded from data analysis.)
CH1_SIG_MEAN
CH1 (green channel, Cy3) mean fluorescence intensity, spot
CH1_BKG_MEAN
CH1 (green channel, Cy3) mean fluorescence intensity, local background
CH2_SIG_MEAN
CH2 (red channel, Cy5) mean fluorescence intensity, spot
CH2_BKG_MEAN
CH2 (red channel, Cy5) mean fluorescence intensity, local background
