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Platform GPL2822 Query DataSets for GPL2822
Status Public on Sep 10, 2005
Title UI Human EST 37K cDNA array
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Homo sapiens
Manufacturer The University of Iowa
Manufacture protocol The clone inserts were amplified directly from bacterial cell cultures with T7 and T3 primers, purified, dried, resuspended in water and printed with 50% DMSO on UltraGaps slides (Corning) using a ProSys 5510 Microarray Printer. The 16 control spikes were printed by each pin, at the beginning or at the end of the pin printing area. The spotted slides were cross-linked and stored in a desiccator.
Support glass
 
Description Glass slide with 37,632 spots (34,462 unique cDNA clone inserts) in 48 blocks consisting of 28 columns and 28 rows. Blocks are arranged in 12 rows and 4 columns.
The set of 34,462 cDNA clone inserts was identified from the University of Iowa EST collection. The clone inserts were amplified with T7 and T3 primers and printed with 50% DMSO on UltraGaps slides (Corning) using a ProSys 5510 Microarray Printer
A small set of full-length clones were ordered from the IMAGE Consortium and spotted on the array.
 
Contributor(s) Koroleva IV, Brown BJ, Snir E, Almabrazi H, Casavant TL, Soares MB
Submission date Sep 08, 2005
Last update date Sep 10, 2005
Contact name Bartley Joseph Brown
E-mail(s) bbrown@eng.uiowa.edu
Phone 319 335 6432
Organization name The University of Iowa
Lab CBCB
Street address 5317
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Samples (43) GSM81060, GSM81061, GSM81062, GSM81063, GSM81149, GSM81150 
Series (1)
GSE3643 Poorly differentiated, human liposarcoma LiSa-2 cell line response to the synthetic triterpenoid CDDO

Data table header descriptions
ID
BLOCK Block of columns and rows within array
COLUMN Column within array, respective to block
ROW Row within array, respective to block
NAME In-house clone name
REARRAY_ID In-house rearrayed clone name, unique per rearray well
GB_ACC GenBank accession number of sequence used to design probe
SPOT_ID identifier for spots without a GenBank Accession

Data table
ID BLOCK COLUMN ROW NAME REARRAY_ID GB_ACC SPOT_ID
1_1_1 1 1 1 UI-X-NAP-aaa-c-02-0-UI LAaaa-e-03 AY722636
1_2_1 1 2 1 UI-X-NAP-aaa-d-03-0-UI LAaaa-f-04 AY722637
1_3_1 1 3 1 UI-X-NBB-aaa-h-08-0-UI LAaaa-c-07 AY722644
1_4_1 1 4 1 UI-X-NAP-aaa-d-06-0-UI LAaaa-d-04 AY722638
1_5_1 1 5 1 UI-X-NBB-aaa-f-05-0-UI LAaaa-h-05 AY722645
1_6_1 1 6 1 UI-X-NXEg-aab-h-06-0-UI LAaaa-d-11 AY722632
1_7_1 1 7 1 UI-X-NAP-aaa-b-02-0-UI LAaaa-f-02 AY722639
1_8_1 1 8 1 UI-X-NAP-aaa-c-08-0-UI LAaaa-h-03 AY722640
1_9_1 1 9 1 UI-X-NAP-aaa-c-10-0-UI LAaaa-h-04 AY722641
1_10_1 1 10 1 UI-X-NS8W-aab-d-02-0-UI LAaaa-c-08 AY722630
1_11_1 1 11 1 UI-X-NS8W-aab-c-10-0-UI LAaaa-d-08 AY722631
1_12_1 1 12 1 UI-X-NAP-aaa-d-09-0-UI LAaaa-a-04 AY722642
1_13_1 1 13 1 None no name given
1_14_1 1 14 1 None no name given
1_15_1 1 15 1 None no name given
1_16_1 1 16 1 None no name given
1_17_1 1 17 1 None no name given
1_18_1 1 18 1 None no name given
1_19_1 1 19 1 None no name given
1_20_1 1 20 1 None no name given

Total number of rows: 37632

Table truncated, full table size 2205 Kbytes.




Download family Format
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Supplementary data files not provided
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