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Status
Public on Dec 30, 2005
Title
IMB Atlantic salmon cDNA microarray
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Salmo salar Manufacturer
Institute for Marine Biosciences/Biotechnology Research Institute
Manufacture protocol
Inserts from 4104 cDNAs were amplified by PCR, purified, resuspended in distilled water and spotted in duplicate on Gap II glass slides in 50% DMSO using a Virtek SDDC-2 ChipWriter at the National Research Council Biotechnology Research Institute. Two-fold dilutions of a plasmid encoding chlorophyll synthetase G4 from Arabidopsis thaliana were included at the bottom of each sub-array to serve as internal controls. The resulting array of 8,208 spots was printed twice on each slide, giving a total of 16,416 spots.
Support
glass
Coating
aminosilane
Description
Corning GAPS II
Web link
http://imb-ibm.nrc-cnrc.gc.ca/projects/salmonmicroarray/index_e.php Contributor(s)
Douglas SE , Ewart K , Tsoi SC , Tessier D , Rigby T Submission date
Sep 13, 2005
Last update date
Dec 30, 2005
Contact name
Jason Williams
E-mail(s)
jason.williams@nrc-cnrc.gc.ca Phone
(902)426-5903
Fax
(902)426-9413
Organization name
National Research Council
Department
Institute for Marine Biosciences
Lab
Microarray
Street address
1411 Oxford Street
City
Halifax
State/province
Nova Scotia
ZIP/Postal code
B3H 3Z1
Country
Canada
Samples (32)
GSM78628 , GSM78629 , GSM78630 , GSM78631 , GSM78632 , GSM78633
GSM78634 ,
GSM78635 ,
GSM268011 ,
GSM268012 ,
GSM268013 ,
GSM268014 ,
GSM268015 ,
GSM268016 ,
GSM268017 ,
GSM268018 ,
GSM268019 ,
GSM268020 ,
GSM268021 ,
GSM268022 ,
GSM268023 ,
GSM268024 ,
GSM268025 ,
GSM268026 ,
GSM268027 ,
GSM268028 ,
GSM268029 ,
GSM268030 ,
GSM268031 ,
GSM268032 ,
GSM268033 ,
GSM268034 Series (2)
GSE3475 Profiling of Salmo salar in two Atlantic salmon types, a wild type versus transgenic.
GSE8526 Salmon Macrophage early response to Aeromonas salmonicida
Data table header descriptions ID
GRID Grid # for array starting at #1 in the top left corner, numbers increase left to right, top to bottom
COLUMN Column number within a subarray (Grid)
ROW Row number within a subarray (Grid)
GENE_ID Gene identification according to library location
SPOT_ID Same information as gene identification
CLONE_ID ORGANISM DESCRIPTION Estimated gene description based on sequence search results
QC
qc Quality control status of print (numeric)
Data table ID GRID COLUMN ROW GENE_ID SPOT_ID CLONE_ID ORGANISM DESCRIPTION QC qc 1
1
1
1
SK1-0320
SK1-0320
SK1-0320 Thyroid receptor interaction protein 15 (trip15)
N/A
N/A
2
1
2
1
SK1-0320
SK1-0320
SK1-0320 Thyroid receptor interaction protein 15 (trip15)
N/A
N/A
3
1
3
1
SK1-0671
SK1-0671
SK1-0671 Centaurin B2 (cntB2)
F
4
4
1
4
1
SK1-0671
SK1-0671
SK1-0671 Centaurin B2 (cntB2)
F
4
5
1
5
1
SK1-0654
SK1-0654
SK1-0654 Thioredoxin (trx)
N/A
N/A
6
1
6
1
SK1-0654
SK1-0654
SK1-0654 Thioredoxin (trx)
N/A
N/A
7
1
7
1
SK1-0284
SK1-0284
SK1-0284 Activating enzyme E1
N/A
N/A
8
1
8
1
SK1-0284
SK1-0284
SK1-0284 Activating enzyme E1
N/A
N/A
9
1
9
1
SK1-0272
SK1-0272
SK1-0272 surface antigen?
W
2
10
1
10
1
SK1-0272
SK1-0272
SK1-0272 surface antigen?
W
2
11
1
11
1
SK1-0622
SK1-0622
SK1-0622 Hybrid arp3/Receptor of activated protein kinase C
N/A
N/A
12
1
12
1
SK1-0622
SK1-0622
SK1-0622 Hybrid arp3/Receptor of activated protein kinase C
N/A
N/A
13
1
13
1
SK1-0614
SK1-0614
SK1-0614 transcription elongation factor
N/A
N/A
14
1
14
1
SK1-0614
SK1-0614
SK1-0614 transcription elongation factor
N/A
N/A
15
1
15
1
SK1-0231
SK1-0231
SK1-0231 Histone deacetylase (hda)
N/A
N/A
16
1
16
1
SK1-0231
SK1-0231
SK1-0231 Histone deacetylase (hda)
N/A
N/A
17
1
17
1
SK1-0223
SK1-0223
SK1-0223 No match
N/A
N/A
18
1
18
1
SK1-0223
SK1-0223
SK1-0223 No match
N/A
N/A
19
1
1
2
SK1-0992
SK1-0992
SK1-0992 EST
G
1
20
1
2
2
SK1-0992
SK1-0992
SK1-0992 EST
G
1
8208 545 Kbytes .Supplementary data files not provided
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