|
| Status |
Public on Dec 30, 2005 |
| Title |
Transgenic Atlantic salmon 12605532b replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mRNA extracted from wild type liver tissue, labelled with Cy5
|
| Organism |
Salmo salar |
| Characteristics |
Wild type Atlantic salmon (control), mRNA extracted from a pooled sample
|
| Biomaterial provider |
Bob Devlin
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
mRNA isolated using Invitrogen's FastTrack 2.0 kit.
|
| Label |
Cy5
|
| |
|
| Channel 2 |
| Source name |
mRNA extracted from Transgenic liver tissue, labelled with Cy3
|
| Organism |
Salmo salar |
| Characteristics |
Transgenic Atlantic salmon (experimental),mRNA extracted from a pooled sample
|
| Biomaterial provider |
Bob Devlin
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
mRNA isolated using Invitrogen's FastTrack 2.0 kit.
|
| Label |
Cy3
|
| |
|
| |
| Hybridization protocol |
Labelled targets were combined (Cy3 and Cy5) with hybridization solution (Amersham hybridization buffer + deionized formamide) and applied to microarray. Microarrays were sealed in diRisi chambers and hybridized at 42C for 16h. After hybridization, microarray slides were washed sequentially with 1X SSC/ 0.2% SDS, 0.1X SSC/0.2% SDS and 0.1 X SSC at 42C before scanning.
|
| Scan protocol |
Scanned on ScanArray 5000XL, Images were quantified with QuantArray (version 3.1)
|
| Description |
Replicate 1 of 4, top half of the array
|
| Data processing |
Lowess NORMALIZATION , data background subtracted. VALUE obtained from NORMALIZED background subtracted ratio of EXP/REF, Log 2 transformed
|
| |
|
| Submission date |
Oct 19, 2005 |
| Last update date |
Nov 23, 2005 |
| Contact name |
Jason Williams |
| E-mail(s) |
jason.williams@nrc-cnrc.gc.ca
|
| Phone |
(902)426-5903
|
| Fax |
(902)426-9413
|
| Organization name |
National Research Council
|
| Department |
Institute for Marine Biosciences
|
| Lab |
Microarray
|
| Street address |
1411 Oxford Street
|
| City |
Halifax |
| State/province |
Nova Scotia |
| ZIP/Postal code |
B3H 3Z1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL2844 |
| Series (1) |
| GSE3475 |
Profiling of Salmo salar in two Atlantic salmon types, a wild type versus transgenic. |
|
