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| Status |
Public on Oct 15, 2007 |
| Title |
OSU RIGUA (Ruminant Immuno-inflammatory Gene Universal Array) 4K |
| Technology type |
spotted oligonucleotide |
| Distribution |
non-commercial |
| Organisms |
Sus scrofa; Bos taurus; Ovis aries |
| Manufacturer |
Edinburgh Research and Innovation, The University of Edinburgh |
| Manufacture protocol |
Researchers at the University of Edinburgh have constructed a microarray to allow investigation into a large set of immuno-inflammatory genes regulated in response to disease, vaccination and therapeutic interventions in ruminants. Specifically designed to investigate the host response to infection by Mycobacterium avium subsp. paratuberculosis (Johne's disease), the microarray can also be applied to other disease states in ruminants. The array will also provide information on selectable traits that may confer resistance to Johne's disease and responses to vaccination. The gene array consists of approximately 600 ruminant immuno-modulatory genes backed up by a database and management system to allow full interrogation of experimental data.
The full array, or RIGUA (Ruminant Immuno-inflammatory Gene Universal Array), consists of some 600 ruminant genes each represented by two non-overlapping 75-mer oligonucleotides. These have been selected to enable investigations into the immune response to infection and other challenges, including vaccination, in ruminants. It will therefore be useful in defining those genes involved in the development of disease, which have an immuno-inflammatory component thus contributing to animal improvement and breeding. It will also be useful in vaccine and adjuvant response studies in the development of vaccines to ruminant disease.
Validation experiments have confirmed the array's applicability to the study of host responses to infection by M. avium subsp. paratuberculosis. Validation has been performed by detailed analysis of sheep with confirmed diagnosis of mycobacterial infection. This analysis has demonstrated the array's power in detecting changes in the regulation of immuno-inflammatory genes in response to infection by Mycobacterium avium subsp. paratuberculosis
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| Description |
For additional information please contact Prof. John Hopkins or S. Rhind at The University of Edinburgh
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| Web link |
http://www.research-innovation.ed.ac.uk/records/opportunities/genemicroarray.asp
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| Contributor(s) |
Ayoubi PJ |
| Submission date |
Oct 16, 2006 |
| Last update date |
Oct 15, 2007 |
| Contact name |
Patricia J Ayoubi |
| E-mail(s) |
ayoubi@okstate.edu
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| Phone |
405-744-6209
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| Fax |
405-744-7799
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| URL |
http://microarray.okstate.edu
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| Organization name |
Oklahoma State University
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| Department |
Biochemistry and Molecular Biology
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| Lab |
OSU Microarray Core Facility
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| Street address |
246 NRC
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| City |
Stillwater |
| State/province |
OK |
| ZIP/Postal code |
74078 |
| Country |
USA |
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| Samples (5) |
GSM140915, GSM140916, GSM140917, GSM259738, GSM259754 |
| Series (2) |
| GSE6083 |
Inflammatory and immune responses in lymph nodes of Iberian red deer infected with Mycobacterium bovis. |
| GSE10286 |
Differential expression of inflammatory and immune response genes in sheep infected with Anaplasma phagocytophilum. |
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