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Platform GPL5460 Query DataSets for GPL5460
Status Public on Oct 10, 2007
Title Genomessage Homo sapiens 0.7k
Technology type spotted DNA/cDNA
Distribution custom-commercial
Organism Homo sapiens
Manufacturer Japan Genome Solutions, Inc
Manufacture protocol For most of the genes, each cDNA was designed approximately 500-600 bp and within approximately 1 kb region from the 3’-poly(A) tail and all cDNAs for microarray probe were cloned into the pGEM vector (Promega, Madison, WI). We also selected and cloned external control genes (approximately 0.5-1 kb) into the pGEM vector to establish the dynamic range of the microarray. Renilla luciferase (RL), baculovirus envelope gp64 (GP), and lambda phage DNA (LD). All clones for capture probe on microarray were sequenced and validated by comparison with the GenBank sequence. The aminosilane surfaces of SuperAmine glass slides (TeleChem International, Sunnyvale, CA) were stamped with quadruply spots of cDNA probe corresponding to each of the capture probe genes with a Geneqs 2000 arrayer (KakenGenex, Matsudo, Japan). The average spot size was 150 mm and separated each other with a distance (500 mm).
 
 
Submission date Jul 02, 2007
Last update date Oct 10, 2007
Contact name Tsutomu Takeuchi
Organization name Saitama Medical University
Department Department of Internal Medicine
Lab Division of Rheumatology/Clinical Immunology
Street address 1981 Tsujido-machi, Kamoda
City Kawagoe-shi, Saitama
ZIP/Postal code 350-8550
Country Japan
 
Samples (74) GSM206911, GSM206912, GSM206913, GSM206914, GSM206915, GSM206916 
Series (2)
GSE8350 mRNA expression profile in peripheral blood cells of RA patients following treatment with an anti-TNFa mAb, infliximab
GSE12926 mRNA profile in purified human peripheral blood eosinophil treated with retinoic acids

Data table header descriptions
ID
CLONE_ ID
Gene Name
GenBank accession #1
GenBank accession #2
GB_LIST GenBank accession numbers
GENE SYMBOL

Data table
ID CLONE_ ID Gene Name GenBank accession #1 GenBank accession #2 GB_LIST GENE SYMBOL
1 JGS_1 Apolipoprotein C-I X00570 NM_001645 X00570, NM_001645 APOC1
2 JGS_2 ATP synthase 6/8 AF347011 AF347011 AF347011, AF347011
3 JGS_3 cytochrome C oxidase 3 BC008342 AF347011 BC008342, AF347011
4 JGS_4 cytochrome C oxidase 2 AF347014 AF347011 AF347014, AF347011
5 JGS_5 alpha-1 antitrypsin; protease inhibitor 1 K01396 NM_000295 K01396, NM_000295 SERPINA1
6 JGS_7 NADH dehydrogenase 4 AF347015 AF347011 AF347015, AF347011
7 JGS_8 alpha-1 acid glycoprotein (Orosomucoid-1) M13692 NM_000607 M13692, NM_000607 ORM1
8 JGS_9 plasma retinol binding protein (RBP) X00129 NM_006744 X00129, NM_006744 RBP4
9 JGS_10 16S ribosomal RNA, mitochondrial AF347015 AF347011 AF347015, AF347011
10 JGS_11 NADH dehydrogenase 3 AF347015 AF347011 AF347015, AF347011
11 JGS_12 cytochrome C oxidase 1 AF347001 AF347011 AF347001, AF347011
12 JGS_13 NADH dehydrogenase 1 BC009345 AF347011 BC009345, AF347011
13 JGS_14 aldolase B X01098 X01098 ALDOB
14 JGS_16 ribosomal protein L41 AF026844 NM_021104 AF026844, NM_021104 RPL41
15 JGS_17 elongation factor 1-alpha (EEF1A1) X16869 NM_001402 X16869, NM_001402 EEF1A1
16 JGS_18 L-alanine:glyoxylate aminotransferase X53414 NM_000030 X53414, NM_000030 AGXT
17 JGS_19 complement factor H (homologue) M65293 NM_002113 M65293, NM_002113 CFH
18 JGS_20 cytochrome P450 IIE1(ethanol-inducible) J02843 NM_000773 J02843, NM_000773 CYP2E1
19 JGS_21 aldehyde dehydrogenase 2 (ALDH2) K03001 NM_000690 K03001, NM_000690 ALDH2
20 JGS_22 alcohol dehydrogenase 4 class II (ADH4) X56419 NM_000670 X56419, NM_000670 ADH4

Total number of rows: 776

Table truncated, full table size 80 Kbytes.






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