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Series GSE8350 Query DataSets for GSE8350
Status Public on Oct 10, 2007
Title mRNA expression profile in peripheral blood cells of RA patients following treatment with an anti-TNFa mAb, infliximab
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Objectives: We monitored the mRNA expression profiles of peripheral blood cells during treatment with a tumor necrosis factor-a inhibitor, infliximab, in patients with rheumatoid arthritis (RA). Using a DNA microarray analysis, we demonstrated a unique set of genes, with distinct baseline and post-treatment changes in expression between responders and non-responders to infliximab treatment.Methods: Using a customized low-density cDNA microarray and a reliable data collection system, we monitored the mRNA expression profiles of whole blood cells from 18 RA patients before and after the infusion of infliximab for up to 22 weeks. The clinical response to treatment with infliximab was determined using the American College of Rheumatology (ACR) response criteria, the disease activity score of 28 joints (DAS28), and individual clinical parameters. The patients were classified as responders or non-responders based on their ACR50% response at 22 weeks. Results: Approximately 15% of the total genes were found to exhibit a greater than 1.5-fold change, compared to their reference values, at one or more time points during the 22 weeks of infliximab therapy. The expression of inflammatory genes, such as interferon-related genes, was strongly correlated with the serum level of C-reactive protein and the DAS28. The increased expression of inflammatory genes in responders was normalized within 2 weeks and then remained at a normal level during the treatment period. In contrast, in the non-responders, the elevated expression at baseline, although it was significantly decreased at 2 weeks, returned to the baseline level after 14 weeks. In addition to inflammatory genes, we identified several groups of genes with distinct differences in expression between the responders and non-responders.Conclusions: Our results suggest that a customized low-density microarray is useful for monitoring mRNA expression profiles in peripheral blood cells, enabling us to identify a unique set of genes with differentially regulated expressions in responders and non-responders to a TNF inhibitor among patients with RA.
Keywords: disease state analysis
 
Overall design 18 RA Patients, We have collected blood samples into PAXgene tube systems for microarray measurement from patients as follows, just before first iv-injection of infliximab, 2 weeks, 14 weeks, and 22 weeks after first infliximab injection.
 
Contributor(s) Sekiguchi N, Kawauchi S, Furuya T, Inaba N, Matsuda K, Ando S, Ogasawara M, Aburatani H, Kameda H, Amano K, Abe T, Ito S, Takeuchi T
Citation(s) 18388148
Submission date Jul 02, 2007
Last update date Mar 17, 2012
Contact name Tsutomu Takeuchi
Organization name Saitama Medical University
Department Department of Internal Medicine
Lab Division of Rheumatology/Clinical Immunology
Street address 1981 Tsujido-machi, Kamoda
City Kawagoe-shi, Saitama
ZIP/Postal code 350-8550
Country Japan
 
Platforms (1)
GPL5460 Genomessage Homo sapiens 0.7k
Samples (72)
GSM206911 Baseline (02-0-0)
GSM206912 Baseline (14-0-0)
GSM206913 Baseline (17-0-0)
Relations
BioProject PRJNA101363

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8350_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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