GEO help: Mouse over screen elements for information.
Status
Public on Apr 01, 2008
Title
WUSTL Human Herpesvirus 1
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Human alphaherpesvirus 1 Manufacturer
Washington University School of Medicine
Manufacture protocol
Oligos were synthesized using standard methods by Illumina (San Diego, CA). These oligonucleotides, as well as Stratagene control A. coli sense oligos #7 and #8 (Ambion part# 1781), were dissolved at a concentration of 50 μM in 3X SSC with 0.75M betaine and were printed in duplicate on MWG Epoxy slides by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/ ).
Support
glass
Coating
other
Description
To construct a gene-specific microarray of the Human Herpesvirus 1 (Herpes simplex virus type 1) genome, we designed 70-mer oligonucleotide array elements. Predicted and known HHV-1 genes were obtained from the strain 17 sequence on the NCBI website (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_001806 ). In addition, genes derived from mouse, common marker and selection genes, and mutant virus sequences were included. Arabidopsis elements were included for normalization. Oligonucleotides were designed from these sequences using ArrayOligoSelector.
Contributor(s)
Alexander DE , Crosby SD , Pasieka T , Leib DA Submission date
Mar 18, 2008
Last update date
Apr 01, 2008
Contact name
Seth Daniel Crosby
E-mail(s)
scrosby@wustl.edu Phone
314-286-1256
Organization name
Washington Univeristy School of Medicine
Department
Genetics
Lab
GTAC
Street address
660 S. Euclid
City
St. Louis
State/province
MO
ZIP/Postal code
63110
Country
USA
Samples (20)
GSM277653 , GSM277654 , GSM277655 , GSM277656 , GSM277657 , GSM277658
GSM277659 ,
GSM277660 ,
GSM277661 ,
GSM277662 ,
GSM277663 ,
GSM277664 ,
GSM277665 ,
GSM277666 ,
GSM277667 ,
GSM277668 ,
GSM277669 ,
GSM277670 ,
GSM277671 ,
GSM277672 Series (1)
GSE10962 Herpes simplex host shutoff attenuates antiviral state
Data table header descriptions ID INTERNAL_SPOT_ID Internal spot id
ORF Genebank Gene
GB_ACC GENOME_ACC Ref Seq Genome Accession
ORGANISM SEQUENCE SPOT_ID
Data table ID INTERNAL_SPOT_ID ORF GB_ACC GENOME_ACC ORGANISM SEQUENCE SPOT_ID 1
Acta1
NM_009606 Mus musculus
GAAGTGCGACATCGACATCAGGAAGGACCTGTATGCCAACAACGTCATGTCAGGGGGCACCACCATGTAC
2
Acta2
NM_007392 Mus musculus
ATGCCTCTGGACGTACAACTGGTATTGTGCTGGACTCTGGAGATGGTGTGACTCACAACGTGCCTATCTA
3
Actb
NM_007393.1 Mus musculus
CTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCG
4
Actg
NM_009609 Mus musculus
CAGGCGGTGCTGTCCTTGTATGCATCTGGGCGCACCACTGGCATTGTCATGGACTCTGGTGACGGGGTCA
5
Adn
NM_013459 Mus musculus
CCTGGGTGCCCACTCCCTGTCCGCCCCTGAACCCTACAAGCGATGGTATGATGTGCAGAGTGTAGTGCCT
6
Akt1
NM_009652 Mus musculus
AGGTCATCGTCGCCAAGGATGAGGTTGCCCACACGCTTACTGAGAACCGTGTCCTGCAGAACTCTAGGCA
7
AL-ORF_36
NC_001806.1
Human herpesvirus 1
GGGGCCCGGAGAGCCGCGGCACCCGGACGCGCCCGGAAAGTCTTTCGCACCACCCGCGATCGGCACGGCC
8
APG10L
NM_025770 Saccharomyces cerevisiae
ATGGAGGATGAGTTCTTTGGAGAAAAAAGCTTCCAGCATTATTGTGCAGAATTCATCAGACATTCACAGC
9
APG12L
NM_026217 Saccharomyces cerevisiae
ATGTCGGAAGATTCAGAGGTTGTGCTGCAGCTCCCCTCGGCTCCTGTAGGAGCCGGCGGCGAGAGCCTTC
10
APG16L
AK005181 Saccharomyces cerevisiae
ATGTCGTCGGGCCTGCGCGCCGCAGACTTTCCCCGCTGGAAGCGTCACATCGCGGAGGAACTGAGGCGCC
11
APG5L
NM_053069 Mus musculus
TGTGCTTCGAGATGTGTGGTTTGGACGAATTCCAACTTGCTTTACTCTCTATCAGGATGAGATAACTGAA
12
APG7L
NM_028835 Mus musculus
TGGAGTTCAGTGCTTTTGACATGAGTGCCTCCACGCCTGCCCACTGCTGCCCGGCCATGGGAACCCTGCA
13
Atf3
NM_007498 Mus musculus
GAAGAGCTGAGATTCGCCATCCAGAATAAACACCTCTGCCATCGGATGTCCTCTGCGCTTGAGTCAGTTA
14
B2m
NM_009735.2 Mus musculus
CCCCCACTGAGACTGATACATACGCCTGCAGAGTTAAGCATGCCAGTATGGCCGAGCCCAAGACCGTCTA
15
Bad
NM_007522 Mus musculus
AGGAAGGCGGTGGAGACCAGCAGCCCAGAGTATGTTCCAGATCCCAGAGTTTGAGCCGAGTGAGCAGGAA
16
Bag2
BC016230 Mus musculus
CCAACCGTCTGATGGGCCGGACCCTCACGGTTGAGGTCTCGGTGGAAACGATCCGAAACCCCCAGCAGGA
17
Bag3
NM_013863 Mus musculus
GTGCCCCCCGAGGGCCCTAAGGACACTGCATCTTCAGCCAACGGCCCTTCCCGCAATGGCTCCCGCCTGC
18
Bag4
NM_026121 Mus musculus
ATGTCGGCCCTGAGGCGCTCGGGCTACGGCCCCAGTGACGGCCCTTCTTACGGCCGCTACTACGGGCCCG
19
bcl2
NM_009741 Mus musculus
TAACGGAGGCTGGGATGCCTTTGTGGAACTATATGGCCCCAGCATGCGACCTCTGTTTGATTTCTCCTGG
20
Becn1
AK007245 Mus musculus
CTGTGAAGGAAGACTTTATGCTCTTGGCAGTTGGAATCATTGCGGCGAGTGCCTTCATCCAAAACCATGA
389 43 Kbytes .Supplementary data files not provided
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