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Status
Public on Nov 17, 2009
Title
NCode 0.24K multi-species miRNA microarray dual color version 2
Technology type
spotted oligonucleotide
Distribution
custom-commercial
Organisms
Caenorhabditis elegans ; Drosophila melanogaster ; Danio rerio ; Homo sapiens ; Mus musculus ; Rattus norvegicus
Manufacturer
Invitrogen
Manufacture protocol
The microarray is contact-printed using microquill pins on Corning® Epoxide-Coated Glass Slides. The array specifications are: Total subarrays per slide: 3, Subarray layout: 8 blocks (4 rows × 2 columns), Block layout: 192 spots (12 rows × 16 columns), Block dimensions: 4 mm × 3 mm Average spot diameter: 120 μm, Spot center-to-center spacing: 265 μm. The array is printed with NCode™ miRNA probes, which are unmodified oligonucleotides (34–44 bases long) containing all known mature miRNAs in the miRBase Sequence Database, Release 9.0, for human, mouse, rat, D. melanogaster, C. elegans, and Zebrafish. Species-specific probes were designed using a proprietary algorithm that generates miRNA sequences with enhanced hybridization properties. The probes provide comparable sensitivity to wild-type sequences, while offering maximum specificity for discerning between closely related miRNAs and normalized melting temperatures for uniform hybridization. Probes have been validated for their ability to distinguish between wild-type and mismatched miRNA sequences with ≥1 base mismatch.
Submission date
Jun 19, 2009
Last update date
Nov 17, 2009
Contact name
Thomas Werge
Phone
+45 4633 4968
Organization name
Psychiatric Center, Sct. Hans Hospital
Department
Institute of biological Psychiatry
Street address
Boserupvej 2
City
Roskilde
ZIP/Postal code
Dk-4000
Country
Denmark
Samples (40)
GSM419055 , GSM419056 , GSM419057 , GSM419058 , GSM419059 , GSM419060
GSM419061 ,
GSM419062 ,
GSM419063 ,
GSM419064 ,
GSM419065 ,
GSM419066 ,
GSM419067 ,
GSM419068 ,
GSM419069 ,
GSM419070 ,
GSM419071 ,
GSM419072 ,
GSM419073 ,
GSM419074 ,
GSM419075 ,
GSM419076 ,
GSM419077 ,
GSM419078 ,
GSM419079 ,
GSM419080 ,
GSM419081 ,
GSM419082 ,
GSM419083 ,
GSM419084 ,
GSM419085 ,
GSM419086 ,
GSM419087 ,
GSM419088 ,
GSM419089 ,
GSM419090 ,
GSM419091 ,
GSM419092 ,
GSM419093 ,
GSM419094
Series (1)
GSE16725
Regional brain expression maps for microRNAs in rats
Data table header descriptions
ID
miRNA_LIST
microRNA name
miRBase
miRBase accession number of miRNA
SEQUENCE
sequence of probe
Data table
ID
miRNA_LIST
miRBase
SEQUENCE
1030
cel-let-7 , hsa-let-7a , rno-let-7a , dre-let-7a
MIMAT0000001 , MIMAT0000062 , MIMAT0000774 , MIMAT0001759
AACTATACAACCTACTACCTCAAACTATACAACCTACTACCTCA
1231
dme-miR-9a , hsa-miR-9 , rno-miR-9 , dre-miR-9
MIMAT0000114 , MIMAT0000441 , MIMAT0000781 , MIMAT0001769
TCATACAGCTAGATAACCAAAGTCATACAGCTAGATAACCAAAG
1431
hsa-let-7b , mmu-let-7b , rno-let-7b , dre-let-7b
MIMAT0000063 , MIMAT0000522 , MIMAT0000775 , MIMAT0001760
AACCACACAACCTACTACCTCAAACCACACAACCTACTACCTCA
1268
hsa-let-7c , mmu-let-7c , rno-let-7c , dre-let-7c
MIMAT0000064 , MIMAT0000523 , MIMAT0000776 , MIMAT0001761
AACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCA
1085
hsa-let-7d , mmu-let-7d , rno-let-7d
MIMAT0000065 , MIMAT0000383 , MIMAT0000562
ACTATGCAACCTACTACCTCTACTATGCAACCTACTACCTCT
1421
hsa-let-7e , mmu-let-7e , rno-let-7e
MIMAT0000066 , MIMAT0000524 , MIMAT0000777
ACTATACAACCTCCTACCTCAACTATACAACCTCCTACCTCA
1033
hsa-let-7f , rno-let-7f , dre-let-7f
MIMAT0000067 , MIMAT0000778 , MIMAT0001764
AACTATACAATCTACTACCTCAAACTATACAATCTACTACCTCA
1064
hsa-miR-100 , mmu-miR-100 , rno-miR-100 , dre-miR-100
MIMAT0000098 , MIMAT0000655 , MIMAT0000822 , MIMAT0001813
CACAAGTTCGGATCTACGGGTCACAAGTTCGGATCTACGGGT
1297
hsa-miR-101 , mmu-miR-101a , rno-miR-101a , dre-miR-101a
MIMAT0000099 , MIMAT0000133 , MIMAT0000823 , MIMAT0001814
CTTCAGTTATCACAGTACTGTACTTCAGTTATCACAGTACTGTA
1164
hsa-miR-103 , mmu-miR-103 , rno-miR-103 , dre-miR-103
MIMAT0000101 , MIMAT0000546 , MIMAT0000824 , MIMAT0001816
TCATAGCCCTGTACAATGCTGTCATAGCCCTGTACAATGCTG
1163
hsa-miR-107 , mmu-miR-107 , rno-miR-107 , dre-miR-107
MIMAT0000104 , MIMAT0000647 , MIMAT0000826 , MIMAT0001817
TGATAGCCCTGTACAATGCTGTGATAGCCCTGTACAATGCTG
1072
hsa-miR-10a , mmu-miR-10a , rno-miR-10a
MIMAT0000253 , MIMAT0000648 , MIMAT0000782
CACAAATTCGGATCTACAGGGTCACAAATTCGGATCTACAGGGT
1213
hsa-miR-124a , rno-miR-124a
MIMAT0000422 , MIMAT0000828
TGGCATTCACCGCGTGCCTTAATGGCATTCACCGCGTGCCTTAA
1377
hsa-miR-127 , rno-miR-127
MIMAT0000446 , MIMAT0000833
AGCCAAGCTCAGACGGATCCGAAGCCAAGCTCAGACGGATCCGA
1384
hsa-miR-139 , mmu-miR-139 , rno-miR-139
MIMAT0000250 , MIMAT0000656 , MIMAT0000845
AGACACGTGCACTGTAGAAGACACGTGCACTGTAGA
1106
hsa-miR-140 , rno-miR-140
MIMAT0000431 , MIMAT0000573
CTACCATAGGGTAAAACCACTCTACCATAGGGTAAAACCACT
1253
hsa-miR-142-3p , rno-miR-142-3p , dre-miR-142a-3p
MIMAT0000434 , MIMAT0000848 , MIMAT0003160
TCCATAAAGTAGGAAACACTACTCCATAAAGTAGGAAACACTAC
1225
hsa-miR-153 , rno-miR-153
MIMAT0000439 , MIMAT0000855
TCACTTTTGTGACTATGCAATCACTTTTGTGACTATGCAA
1272
hsa-miR-16 , mmu-miR-16 , rno-miR-16
MIMAT0000069 , MIMAT0000527 , MIMAT0000785
CGCCAATATTTACGTGCTGCTACGCCAATATTTACGTGCTGCTA
1031
hsa-miR-17-5p , mmu-miR-17-5p , rno-miR-17
MIMAT0000070 , MIMAT0000649 , MIMAT0000786
ACTACCTGCACTGTAAGCACTTACTACCTGCACTGTAAGCACTT
Total number of rows: 236 Table truncated, full table size 27 Kbytes .
Supplementary data files not provided