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Platform GPL8745 Query DataSets for GPL8745
Status Public on Nov 17, 2009
Title NCode 0.24K multi-species miRNA microarray dual color version 2
Technology type spotted oligonucleotide
Distribution custom-commercial
Organisms Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Homo sapiens; Mus musculus; Rattus norvegicus
Manufacturer Invitrogen
Manufacture protocol The microarray is contact-printed using microquill pins on Corning® Epoxide-Coated Glass Slides. The array specifications are: Total subarrays per slide: 3, Subarray layout: 8 blocks (4 rows × 2 columns), Block layout: 192 spots (12 rows × 16 columns), Block dimensions: 4 mm × 3 mm
Average spot diameter: 120 μm, Spot center-to-center spacing: 265 μm. The array is printed with NCode™ miRNA probes, which are unmodified oligonucleotides (34–44 bases long) containing all known mature miRNAs in the miRBase Sequence Database, Release 9.0, for human, mouse, rat, D. melanogaster, C. elegans, and Zebrafish. Species-specific probes were designed using a proprietary algorithm that generates miRNA sequences with enhanced hybridization properties. The probes provide comparable sensitivity to wild-type sequences, while offering maximum specificity for discerning between closely related miRNAs and normalized melting temperatures for uniform hybridization. Probes have been validated for their ability to distinguish between wild-type and mismatched miRNA sequences with ≥1 base mismatch.
 
 
Submission date Jun 19, 2009
Last update date Nov 17, 2009
Contact name Thomas Werge
Phone +45 4633 4968
Organization name Psychiatric Center, Sct. Hans Hospital
Department Institute of biological Psychiatry
Street address Boserupvej 2
City Roskilde
ZIP/Postal code Dk-4000
Country Denmark
 
Samples (40) GSM419055, GSM419056, GSM419057, GSM419058, GSM419059, GSM419060 
Series (1)
GSE16725 Regional brain expression maps for microRNAs in rats

Data table header descriptions
ID
miRNA_LIST microRNA name
miRBase miRBase accession number of miRNA
SEQUENCE sequence of probe

Data table
ID miRNA_LIST miRBase SEQUENCE
1030 cel-let-7, hsa-let-7a, rno-let-7a, dre-let-7a MIMAT0000001, MIMAT0000062, MIMAT0000774, MIMAT0001759 AACTATACAACCTACTACCTCAAACTATACAACCTACTACCTCA
1231 dme-miR-9a, hsa-miR-9, rno-miR-9, dre-miR-9 MIMAT0000114, MIMAT0000441, MIMAT0000781, MIMAT0001769 TCATACAGCTAGATAACCAAAGTCATACAGCTAGATAACCAAAG
1431 hsa-let-7b, mmu-let-7b, rno-let-7b, dre-let-7b MIMAT0000063, MIMAT0000522, MIMAT0000775, MIMAT0001760 AACCACACAACCTACTACCTCAAACCACACAACCTACTACCTCA
1268 hsa-let-7c, mmu-let-7c, rno-let-7c, dre-let-7c MIMAT0000064, MIMAT0000523, MIMAT0000776, MIMAT0001761 AACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCA
1085 hsa-let-7d, mmu-let-7d, rno-let-7d MIMAT0000065, MIMAT0000383, MIMAT0000562 ACTATGCAACCTACTACCTCTACTATGCAACCTACTACCTCT
1421 hsa-let-7e, mmu-let-7e, rno-let-7e MIMAT0000066, MIMAT0000524, MIMAT0000777 ACTATACAACCTCCTACCTCAACTATACAACCTCCTACCTCA
1033 hsa-let-7f, rno-let-7f, dre-let-7f MIMAT0000067, MIMAT0000778, MIMAT0001764 AACTATACAATCTACTACCTCAAACTATACAATCTACTACCTCA
1064 hsa-miR-100, mmu-miR-100, rno-miR-100, dre-miR-100 MIMAT0000098, MIMAT0000655, MIMAT0000822, MIMAT0001813 CACAAGTTCGGATCTACGGGTCACAAGTTCGGATCTACGGGT
1297 hsa-miR-101, mmu-miR-101a, rno-miR-101a, dre-miR-101a MIMAT0000099, MIMAT0000133, MIMAT0000823, MIMAT0001814 CTTCAGTTATCACAGTACTGTACTTCAGTTATCACAGTACTGTA
1164 hsa-miR-103, mmu-miR-103, rno-miR-103, dre-miR-103 MIMAT0000101, MIMAT0000546, MIMAT0000824, MIMAT0001816 TCATAGCCCTGTACAATGCTGTCATAGCCCTGTACAATGCTG
1163 hsa-miR-107, mmu-miR-107, rno-miR-107, dre-miR-107 MIMAT0000104, MIMAT0000647, MIMAT0000826, MIMAT0001817 TGATAGCCCTGTACAATGCTGTGATAGCCCTGTACAATGCTG
1072 hsa-miR-10a, mmu-miR-10a, rno-miR-10a MIMAT0000253, MIMAT0000648, MIMAT0000782 CACAAATTCGGATCTACAGGGTCACAAATTCGGATCTACAGGGT
1213 hsa-miR-124a, rno-miR-124a MIMAT0000422, MIMAT0000828 TGGCATTCACCGCGTGCCTTAATGGCATTCACCGCGTGCCTTAA
1377 hsa-miR-127, rno-miR-127 MIMAT0000446, MIMAT0000833 AGCCAAGCTCAGACGGATCCGAAGCCAAGCTCAGACGGATCCGA
1384 hsa-miR-139, mmu-miR-139, rno-miR-139 MIMAT0000250, MIMAT0000656, MIMAT0000845 AGACACGTGCACTGTAGAAGACACGTGCACTGTAGA
1106 hsa-miR-140, rno-miR-140 MIMAT0000431, MIMAT0000573 CTACCATAGGGTAAAACCACTCTACCATAGGGTAAAACCACT
1253 hsa-miR-142-3p, rno-miR-142-3p, dre-miR-142a-3p MIMAT0000434, MIMAT0000848, MIMAT0003160 TCCATAAAGTAGGAAACACTACTCCATAAAGTAGGAAACACTAC
1225 hsa-miR-153, rno-miR-153 MIMAT0000439, MIMAT0000855 TCACTTTTGTGACTATGCAATCACTTTTGTGACTATGCAA
1272 hsa-miR-16, mmu-miR-16, rno-miR-16 MIMAT0000069, MIMAT0000527, MIMAT0000785 CGCCAATATTTACGTGCTGCTACGCCAATATTTACGTGCTGCTA
1031 hsa-miR-17-5p, mmu-miR-17-5p, rno-miR-17 MIMAT0000070, MIMAT0000649, MIMAT0000786 ACTACCTGCACTGTAAGCACTTACTACCTGCACTGTAAGCACTT

Total number of rows: 236

Table truncated, full table size 27 Kbytes.




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