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Platform GPL9106 Query DataSets for GPL9106
Status Public on Jun 01, 2010
Title MRC Toxicology Unit, Mus musculus, oligo MEEBO microarrays
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mus musculus
Manufacturer MRC Toxicology Unit, Systems Toxicology Group
Manufacture protocol 1.Oligos are in 101 384 well plates (MEEBO set) (Cat OL -10-122). Each well contains 200 pmol of 70-mer oligo.  Print split over 2 slides – A and B.
2. Resuspend oligos in 20ul printing buffer to 10uM.  Printing buffer is 1.5M betaine, 3xSSC.
3. Seal plates with adhesive PCR film from Applied Biosystems (cat. AB-0558).
4. Shake plates on a multi plate shaker for about 15 minutes This resuspends the dried down oligo in the printing buffer.
5. Centrifuge plates at 3000rpm for 5 minutes.
6. Remove seal immediately prior to use. Place one plate at a time in appropriate location of plate holder on arrayer.  Screw to keep in position.  Attach 32 pin head to standford design arrayer and start print run.  After all 384 samples have been printed software will request next plate be placed in position.  Remove previous plate, reseal and replace with next plate.
 
 
Submission date Aug 26, 2009
Last update date Jan 18, 2013
Contact name Amy Pointon
Organization name MRC Toxicology Unit
Department Systems Toxicology Group
Street address Lancaster Road
City Leicester
ZIP/Postal code LE1 9NH
Country United Kingdom
 
Samples (149) GSM444703, GSM444704, GSM444705, GSM444706, GSM444707, GSM444708 
Series (6)
GSE17823 Mouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 15mg/kg doxorubicin
GSE17826 Mouse cardiac tissue: Vehicle treated control vs. 15mg/kg doxorubicin
GSE17830 Mouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 25mg/kg DMNQ

Data table header descriptions
ID Oligo ID from probe set
GB_LIST NCBI Accession number
ORF Gene symbols
Array Name Oligo probe name
SPOT_ID spot identifier

Data table
ID GB_LIST ORF Array Name SPOT_ID
EMPTY 003-MCC-- EMPTY
mCA000101 NM_007393 Actb 001-CTRL-NM_007393-Actb
mCA000111 NM_011740 Ywhaz 001-CTRL-NM_011740-Ywhaz
mCA000163 NM_007393 Actb 001-CTRL-NM_007393-Actb
mCA000221 NM_007907 Eef2 001-CTRL-NM_007907-Eef2
mCA000230 NM_009983 Ctsd 001-CTRL-NM_009983-Ctsd
mCA000240 NM_009089 Polr2a 001-CTRL-NM_009089-Polr2a
mCA000295 NM_011602 Tln 001-CTRL-NM_011602-Tln
mCA000303 NM_019642 Rpn2 001-CTRL-NM_019642-Rpn2
mCA000312 NM_009983 Ctsd 001-CTRL-NM_009983-Ctsd
mCA000356 NM_013556 Hprt 001-CTRL-NM_013556-Hprt
mCA000366 NM_007907 Eef2 001-CTRL-NM_007907-Eef2
mCA000438 NM_009438 Rpl13a 002-CTRL-NM_009438-Rpl13a
mCA000439 BC031849 Sdha 002-CTRL-BC031849-Sdha
mCA000442 NM_019639 Ubc 002-CTRL-NM_019639-Ubc
mCA000452 NM_008084 LOC14433 002-CTRL-NM_008084-LOC14433
mCA000455 NM_009438 Rpl13a 002-CTRL-NM_009438-Rpl13a
mCA000460 NM_013684 Tbp 002-CTRL-NM_013684-Tbp
mCA000474 NM_008512 Lrp1 002-CTRL-NM_008512-Lrp1
mCA000483 NM_019642 Rpn2 002-CTRL-NM_019642-Rpn2

Total number of rows: 38468

Table truncated, full table size 1946 Kbytes.




Download family Format
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Supplementary data files not provided

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