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| Status |
Public on Aug 06, 2010 |
| Title |
Mouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 15mg/kg doxorubicin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Translational profiling of mouse cardiac tissue treated with 15mg/kg doxorubicin in 10 ml/kg saline over an acute time course (0.5-120 hours) compared to time matched control animals treated with 10ml/kg saline.
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| Overall design |
Two colour microarrays with time matched controls vs 15mg/kg doxorubicin cardiac tissue. Before microarray analysis, RNA is separated on a sucrose density gradient into those mRNAs activity undergoing translation (polysomes) and those not (monosomes) with control monosomes and treated monosomes on one set of arrays, and polysome control and polysome treated on another set of microarrays. Thealized Log2 of the monosomes was subtracted from the respective Log2 of the polysomes (on Series record). Time points studied were 0.5, 1, 2, 12, 24 and 120 hours following dosing, biological replicates n=3 independent animals at each time point, technical replicates (reverse labelling) n<1. One array printed onto two slides (A and B), one replicate per array.
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| Contributor(s) |
Pointon A |
| Citation(s) |
20856801 |
| |
| Submission date |
Aug 26, 2009 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Amy Pointon |
| Organization name |
MRC Toxicology Unit
|
| Department |
Systems Toxicology Group
|
| Street address |
Lancaster Road
|
| City |
Leicester |
| ZIP/Postal code |
LE1 9NH |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL9106 |
MRC Toxicology Unit, Mus musculus, oligo MEEBO microarrays |
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| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE18459 |
Mouse cardiac tissues treated with doxorubicin and DMNQ |
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| Relations |
| BioProject |
PRJNA123299 |
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