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| Status |
Public on Aug 25, 2017 |
| Title |
Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies VII |
| Platform organisms |
Quercus alba; Gossypium hirsutum; Rhodotorula; Trypanosoma cruzi; Pandalus borealis; Oncorhynchus nerka; Gallus gallus; Homo sapiens; Sus scrofa domesticus; Bos taurus; Ovis aries; Oryctolagus cuniculus; Mus musculus; Human immunodeficiency virus 1; Ceratonia siliqua; Megathura crenulata; Bothrops moojeni; Frangula alnus; Senegalia senegal; Pachymenia carnosa; Dermestes lardarius |
| Sample organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
|
| Summary |
Purpose: This study uses a high-throughput glycan microarray to evaluate the immunological evolution of antibodies to the glyco-antigen GD2. The goal is to determine germline and affinity mature antibody specificity and affinities/
Results: Affinity mature anti-GD2 antibodies 3F8 and ch14.18 had high affinity and were highly specific for the target GD2. Germline antibodies were also hihgly specific and had surprisingly high affinity.
Conclusion: Antibodies to GD2 evolved from highly specific germlines. Highly specific germlines may be critical in evading autoimmunity issues.
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| Overall design |
Germline variable regions of 3F8 and ch14.18 were determined. Germline and affinity mature antibody DNA were synthesized and transfected into HEK293 cell lines. Secreted antibodies were purified from supernatant using affinity chromatography. Antibodies were evaluated on multiple assay platforms, including glycan microarrays, protein microarrays, and cell binding assays.
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| Contributor(s) |
Sterner E, Peach ML, Nicklaus MC, Gildersleeve JC |
| Citation(s) |
28813678 |
| Submission date |
Jun 21, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Jeff Gildersleeve |
| Organization name |
National Cancer Institute
|
| Street address |
376 Boyles St.
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
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| Samples (7)
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| GSM2677672 |
Humanized 3F8 -- Anti-GD2 Antibody hu3F8_1 |
| GSM2677673 |
Humanized 3F8 -- Anti-GD2 Antibody hu3F8_2 |
| GSM2677674 |
Humanized 3F8 -- Anti-GD2 Antibody hu3F8_3 |
| GSM2677675 |
Humanized 3F8 -- Anti-GD2 Antibody hu3F8_4 |
| GSM2677676 |
Humanized 3F8 -- Anti-GD2 Antibody hu3F8_5 |
| GSM2677677 |
Humanized 3F8 -- Anti-GD2 Antibody hu3F8_6 |
| GSM2677678 |
Humanized 3F8 -- Anti-GD2 Antibody hu3F8_7 |
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| This SubSeries is part of SuperSeries: |
| GSE100438 |
Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies |
|
| Relations |
| BioProject |
PRJNA391739 |
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