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| Status |
Public on Dec 01, 2017 |
| Title |
Effect of L-tryptophan, tryptamine and indole-3 acetaldehyde on gene expression in Fusarium graminearum |
| Organism |
Fusarium graminearum |
| Experiment type |
Expression profiling by array
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| Summary |
Fusarium graminearum is a plant pathogen that can cause the devastating cereal grain disease fusarium head blight (FHB) in temperate regions of the world. Previous studies have shown that F. graminearum can synthetize indole-3-acetic acid (auxin) using L-tryptophan (L-TRP)-dependent pathways. In the present study, Gene expression profiles were obtained using microarray analysis of RNAs from F. graminearum cultures in auxin producing conditions, treated with L-TRP, tryptamine (TAM) and indole-3 acetaldehyde (IAAld). A comparative expression profiling of all treatments identified candidate genes for auxin production in F. graminearum. Additional analysis of the expression profiling between L-TRP-treated and control cultures showed that L-TRP treatment induce the up-regulation of a series of genes with predicted function in the metabolism of L-TRP via anthranilic acid and catechol towards the tricarboxylic acid cycle.
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| Overall design |
F. graminearum stain DAOM180378 mycelia samples in 2nd stage media were treated with either 2 mM L-TRP, 0.2 mM TAM, 0.2 mM IAAld or were untreated (NT). Dual-channel, three biological replicated for each treatment with two technical replicates (dye-swaps) for each sample.
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| Web link |
http://hdl.handle.net/10393/23130
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| Contributor(s) |
DesRoches C, Johnston A, Luo K, Harris LJ, Zhao H, Ouellet T |
| Citation(s) |
28926717 |
| |
| Submission date |
Jun 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Linda J Harris |
| E-mail(s) |
Linda.Harris@agr.gc.ca
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| Phone |
613-759-1314
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| Organization name |
Agriculture & Agri-Food Canada (AAFC)
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| Department |
Eastern Cereal & Oilseed Research Centre
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| Lab |
Building #21
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| Street address |
960 Carling Ave.
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| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1A 0C6 |
| Country |
Canada |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA391306 |
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