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| Status |
Public on Jun 27, 2017 |
| Title |
High-resolution mapping of heteroduplex DNA formed in UV-induced and spontaneous mitotic recombination events in yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome variation profiling by SNP array
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| Summary |
In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination.
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| Overall design |
Using whole genome and chromosome-specific SNP microarray, we mapped heteroduplex DNA, a recombination intermediate, from all four granddaughter cells after spontaneous or UV-induced mitotic recombination events. We also determined linkage of SNPs by mapping spores derived from these granddaughter cells.
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| Contributor(s) |
Yin Y, Petes T |
| Citation(s) |
28714850 |
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| Submission date |
Jun 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yi Yin |
| E-mail(s) |
yy224@uw.edu
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| Organization name |
University of Washington
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| Department |
Genome Sciences
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| Lab |
Shendure Lab
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| Street address |
3720 15th Ave NE
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| City |
Seattle |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (3) |
| GPL20144 |
Agilent-027438 Yeast S-Y SNP v1 [Probe Name Version] |
| GPL21274 |
Agilent-043262 ch5array 031671 [Feature Number Version] |
| GPL21553 |
Agilent-031671 Chr4 RA V2 027289 (SPOT_ID version) |
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| Samples (144)
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| Relations |
| BioProject |
PRJNA391954 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100497_RAW.tar |
745.6 Mb |
(http)(custom) |
TAR (of GPR, TXT) |
| Processed data provided as supplementary file |
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