|
Status |
Public on Nov 25, 2008 |
Title |
Effect of TCDD Exposure on purified splenic CD8 T cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
8-12 week, female C57BL/6 mice were injected with 10 µg/kg TCDD or solvent control. CD8+ T cells from spleen were FACS purified and submitted to transcription profiling Keywords: TCDD, AHR, Immunosupression peripheral T cells, CD8 T cells, Toxicogenomics
|
|
|
Overall design |
For each group 4 female C57BL/6 mice were treated, spleen surgically removed and single cell suspensions generated. Cell suspensions were pooled, and CD8 positive T cells purified (purity > 99%). RNA was isolated using TRIzol and amplified using the Ambion message AMP kit. Target lableing and hybridization was performed using Affymetrix standard protocols
|
|
|
Contributor(s) |
Frericks M, Esser C |
Citation(s) |
16972790 |
|
Submission date |
Jan 07, 2008 |
Last update date |
Jan 08, 2019 |
Contact name |
Markus Frericks |
E-mail(s) |
fremar5@web.de
|
Phone |
+49(0)211 3389253
|
Fax |
+49(0)211 3190910
|
Organization name |
Institute for Environmental Research
|
Department |
Immunology
|
Street address |
Auf'm Hennekamp 50
|
City |
Duesseldorf |
ZIP/Postal code |
40225 |
Country |
Germany |
|
|
Platforms (1) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
|
Samples (4)
|
|
Relations |
BioProject |
PRJNA108661 |