|
| Status |
Public on Jan 01, 2018 |
| Title |
Expression data fron HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The protein GIGYF2 is an RNA-binding protein that mediates repression of mRNA function when tethered to an artificial reporter mRNA. Cellular targets of GIGYF2 were not known at the time of this study.
We used microarrays to globally identify gene expression changes upon manipulating GIGYF2 expression levels.
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| Overall design |
HeLa cells were either transfected with a control plasmid or a plasmid coding for GIGYF2 under a CMV promoter, and then processed for RNA extraction and hybridization on Affymetrix microarrays. For each condition (WT or overexpression) three biological replicate samples were prepared. Microarray analysis was performed at the EMBL GeneCore facility (Heidelberg, Germany)
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| |
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| Contributor(s) |
Béthune J, Ramirez CA |
| Citation(s) |
29554310 |
| |
| Submission date |
Jul 10, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Julien Béthune |
| E-mail(s) |
julien.bethune@bzh.uni-heidelberg.de
|
| Phone |
+49 6221 54 4749
|
| Organization name |
Heidelberg University Biochemistry Center
|
| Lab |
Béthune Lab
|
| Street address |
Im Neuenheimerfeld 328
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM2699151 |
Control GIGY2-overexpressing HeLa cells biological rep 1 |
| GSM2699152 |
Control GIGY2-overexpressing HeLa cells biological rep 2 |
| GSM2699153 |
Control GIGY2-overexpressing HeLa cells biological rep 3 |
|
| Relations |
| BioProject |
PRJNA393710 |
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