|
| Status |
Public on Jan 01, 2018 |
| Title |
Control GIGY2-overexpressing HeLa cells biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells 24h post transfection
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
transfection: plasmid coding for GIGYF2 under a CMV promoter
|
| Treatment protocol |
Cells were transfected with PEI 24h before RNA extraction
|
| Growth protocol |
HeLa cells were grown in DMEM medium containing 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRI reagentl extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng ug total RNA (Ambion WT Expression kit, catalog number 4411974 and Affymetrix GeneChip® WT Terminal Labeling and Controls Kit, catalogue number 901524).
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45°C to Affymetrix Human Gene 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
Gene expression data from GIGYF2-overexpressing cells
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) Software (version 4.0) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Jul 10, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Julien Béthune |
| E-mail(s) |
julien.bethune@bzh.uni-heidelberg.de
|
| Phone |
+49 6221 54 4749
|
| Organization name |
Heidelberg University Biochemistry Center
|
| Lab |
Béthune Lab
|
| Street address |
Im Neuenheimerfeld 328
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
|
