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| Status |
Public on Jul 25, 2017 |
| Title |
Transcriptomic analysis of a novel Saccharomyces cerevisiae strain with improved xylose fermentation ability under heat and acid co-stress |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Xylose-utilizing yeasts with tolerances to fermentation inhibitors (such as weak organic acids) and high temperature are needed for cost-effective simultaneous saccharification and co-fermentation (SSCF) of lignocellulosic materials. We constructed a novel xylose-assimilating Saccharomyces cerevisiae strain with improved fermentation performance under heat and acid co-stress using the genome shuffling technique. Two xylose-utilizing diploid yeasts with different genetic backgrounds were used as the parental strains for genome shuffling. The hybrid strain Hyb-8 showed significantly higher xylose fermentation ability than both parental strains (Sun049T-Z and Sun224T-K) under co-stress conditions of heat and acids. To screen for genes that might be important for fermentation under heat and acid co-stress, a transcriptomic analysis of hybrid strain Hyb-8 and its parental strains was performed.
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| Overall design |
Total RNA extracted from three Saccharomyces cerevisiae strains (Sun049T-Z, Sun224T-K, and Hyb-8) was used for the transcriptomic analysis by Agilent microarray. Each microarray sample was analyzed in triplicate resulting in total of 9 samples.
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| Contributor(s) |
Inokuma K, Iwamoto R, Hasunuma T, Kondo A |
| Citation(s) |
29326929 |
| |
| Submission date |
Jul 24, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Akihiko Kondo |
| Organization name |
Kobe University
|
| Department |
Chemical Science and Engineering
|
| Street address |
1-1 Rokkodai, Nada
|
| City |
Kobe |
| ZIP/Postal code |
657-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL9294 |
Agilent-015072 Yeast Oligo Microarray 4x44K G2519F (Probe Name version) |
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| Samples (9)
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| GSM2715514 |
Thermotolerant_Saccharomyces_cerevisiae_Sun049T-Z_replicate 1 |
| GSM2715515 |
Thermotolerant_Saccharomyces_cerevisiae_Sun049T-Z_replicate 2 |
| GSM2715516 |
Thermotolerant_Saccharomyces_cerevisiae_Sun049T-Z_replicate 3 |
| GSM2715517 |
Acid_tolerant_Saccharomyces_cerevisiae_Sun224T-K_replicate 1 |
| GSM2715518 |
Acid_tolerant_Saccharomyces_cerevisiae_Sun224T-K_replicate 2 |
| GSM2715519 |
Acid_tolerant_Saccharomyces_cerevisiae_Sun224T-K_replicate 3 |
| GSM2715520 |
Hybrid_Saccharomyces_cerevisiae_Hyb-8_replicate 1 |
| GSM2715521 |
Hybrid_Saccharomyces_cerevisiae_Hyb-8_replicate 2 |
| GSM2715522 |
Hybrid_Saccharomyces_cerevisiae_Hyb-8_replicate 3 |
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| Relations |
| BioProject |
PRJNA395510 |
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