|
| Status |
Public on Feb 25, 2008 |
| Title |
High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and polIV mutants |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Comparison of the endogenous small RNA content of Arabidopsis flower bud tissue: wild type vs. mutants in polIV pathways
Keywords: High throughput 454 small RNA sequencing.
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|
| Overall design |
Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to 454 high throughput pyrosequencing. Please see www.454.com for details of the sequencing technology.
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| |
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| Contributor(s) |
Mosher RA, Schwach F, Studholme DJ, Baulcombe DC |
| Citation(s) |
18287047 |
| |
| Submission date |
Jan 15, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Frank Schwach |
| E-mail(s) |
f.schwach@uea.ac.uk
|
| Organization name |
University of East Anglia
|
| Department |
School of Computing Sciences
|
| Lab |
Computational Biology Group
|
| Street address |
University of East Anglia
|
| City |
Norwich |
| ZIP/Postal code |
NR4 7UH |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL6375 |
High-throughput pyrosequencing of small RNAs in Arabidopsis thaliana |
|
| Samples (3) |
| GSM257235 |
endogenous small RNAs from Arabidopsis thaliana col-0, wild type, flower buds |
| GSM257236 |
endogenous small RNAs from Arabidopsis thaliana col-0, nrpd1a mutant, flower buds |
| GSM257237 |
endogenous small RNAs from Arabidopsis thaliana col-0, nrpd1b mutant, flower buds |
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| Relations |
| BioProject |
PRJNA108391 |
