|
| Status |
Public on Sep 01, 2008 |
| Title |
L. lactis ΔlmrCD and cholate-adapted L. lactis ΔlmrCDR |
| Platform organism |
Lactococcus cremoris subsp. cremoris MG1363 |
| Sample organism |
Lactococcus lactis |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptome profiling of a L. lactis strain lacking the MDR transporter genes lmrCD and the same strain adapted to cholate was performed. As compared to the parental strain, the cholate-adapted strain showed 124 genes that were significantly differentially expressed more than 1.8-fold . These genes could be grouped into four major functional classes: i.e., genes involved in (i) cell envelope biogenesis; (ii) stress response and molecular chaperones; (iii) general metabolism and house-keeping function; and (iv) the chromosomally embedded sex-factor.
Keywords: Mutant versus wild type comparison, stress response, cholate
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| |
|
| Overall design |
1. WT1 L. lactis ΔlmrCD (labeled with Cy3)
2. WT2 L. lactis ΔlmrCD (labeled with Cy5)
3. WT4 L. lactis ΔlmrCD (labeled with Cy3)
4. WT5 L. lactis ΔlmrCD (labeled with Cy5)
5. M1 L. lactis ΔlmrCD(R) (labeled with Cy5)
6. M2 L. lactis ΔlmrCD(R)(labeled with Cy3)
7. M4 L. lactis ΔlmrCD(R) (labeled with Cy5)
8. M5 L. lactis ΔlmrCD(R) (labeled with Cy3)
Hybridization schema:
1+5 (Slide 176631)
3+7 (Slide 176632)
2+8 (Slide 176633)
4+6 (Slide 176634)
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| |
|
| Citation(s) |
18790870 |
| |
| Submission date |
Jan 18, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Anne de Jong |
| E-mail(s) |
anne.de.jong@rug.nl
|
| Phone |
+31 50 363 2047
|
| Organization name |
university of Groningen
|
| Department |
Molecular Genetics
|
| Street address |
Nijenborgh 7
|
| City |
Groningen |
| ZIP/Postal code |
9747 AG |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL5048 |
University of Groningen Lactococcus lactis MG1363 amplicon 5K |
|
| Samples (4)
|
| GSM257688 |
176631 - L. lactis ΔlmrCD and cholate-adapted L. lactis ΔlmrCDR |
| GSM257690 |
176632 - L. lactis ΔlmrCD and cholate-adapted L. lactis ΔlmrCDR |
| GSM257691 |
176633 - L. lactis ΔlmrCD and cholate-adapted L. lactis ΔlmrCDR |
| GSM257692 |
176634 - L. lactis ΔlmrCD and cholate-adapted L. lactis ΔlmrCDR |
|
| Relations |
| BioProject |
PRJNA108321 |
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