|
| Status |
Public on Apr 08, 2018 |
| Title |
NANOG is required for the long-term establishment of avian somatic reprogrammed cells [chicken] |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: the goal of this study is to analyse various chicken stem cell lines and to compare them with both primary somatic fibroblasts and blastodermal cells derived from pre-gastrulating embryos
Methods: NGS RNA-sequencing was performed on chicken primary embryonic fibroblasts (CEF), embryonic stem cells (cESC), on blastodermal cells of EGK-X EGK-XII stages embryos (BCs), long term in vitro cultured primordial germ cells (PGCs) as specific chicken stem cells and reprogrammed cells (1A, 1D, 3E, 3F) derived from CEF by somatic reprogramming process. The librairies were prepared using the TruSeq R Stranded mRNA sample preparation kit (Illumina). The paired-end sequencing was performed on the NextSeq 500 sequencer (Illumina) and controlled by the Seqencing Analysis viewer software (Illumina). The quality control of the Sample-ID.fastq files was performed with the FasQC software (Babraham Institute).
Results: the total number of reads ranges from was of 80 millions to 145 millions.
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| |
|
| Overall design |
Chicken stem cells and somatic cells mRNA profiles were generated by Next-generation sequencing (NGS) using Illumina kits
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| |
|
| Contributor(s) |
Sylvain-Vidal V, Vidal V, Jean C, Pain B |
| Citation(s) |
30318291 |
| |
| Submission date |
Aug 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Bertrand Pierre-Yves PAIN |
| E-mail(s) |
bertrand.pain@inserm.fr
|
| Phone |
33632513528
|
| Organization name |
U1208, INSERM
|
| Street address |
18, Avenue du Doyen Lépine
|
| City |
Bron |
| ZIP/Postal code |
69675 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
|
| Samples (9)
|
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| This SubSeries is part of SuperSeries: |
| GSE109970 |
NANOG is required for the long-term establishment of avian somatic reprogrammed cells |
|
| Relations |
| BioProject |
PRJNA397544 |
| SRA |
SRP115012 |
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