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| Status |
Public on Nov 03, 2009 |
| Title |
A Global Genomic View of MIF Knockdown Cell |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
siRNA-mediated knockdown of MIF expression in HEK293 cells resulted in inhibition of cell proliferation and cell cycle progress. The microarray study of MIF KD cells reveald knockdown of MIF would lead to extensive changes in gene expression profiles which may elucidate the molecular mechanism of MIF siRNA-mediated inhibition of cell cycle and cell proliferation.
Keywords: The whole-genome expression analysis in MIF knockdown cells and the control cells
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| Overall design |
HEK293 cells were transfected MIF siRNA (50pmol/ml & 100pmol/ml) or control RNA using LipofectamineTM 2000 reagent and re-incubated for 24 hours.At the designed time point, totol RNA was isolated from the different treated cells.The normal cells were used as control.
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| |
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| Contributor(s) |
Liu L |
| Citation(s) |
18469521 |
| Submission date |
Jan 23, 2008 |
| Last update date |
Feb 15, 2013 |
| Contact name |
Lingfeng Liu |
| E-mail(s) |
liulingfeng1111@gmail.com
|
| Phone |
+86-21-65643262
|
| Organization name |
Fudan University
|
| Department |
Life Science
|
| Lab |
the State Key Laboratory of Genetic Engineering
|
| Street address |
Handan Road 220#
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL6102 |
Illumina human-6 v2.0 expression beadchip |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA108421 |
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