|
| Status |
Public on Nov 03, 2009 |
| Title |
HEK293_MIF siRNA 2#_24h_Illumina |
| Sample type |
RNA |
| |
|
| Source name |
HEK293 cell, MIF Knockdown
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293 human embryonic kidney cell line
protocol: MIF depletion, siRNA-mediated
siRNA concentration = 50pmol/ml
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Illumina TotalPrep RNA amplification kit (Ambion Catalog#IL1791)
|
| Label |
Streptavidin Cy3
|
| Label protocol |
As described in the illumina Human-6 chip processing protocol. Briefly, generation of biotin labelled cRNA from the product of RT cDNA synthesis and 2nd strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
Refer to the Illumina Gene Expression System Manual
|
| Scan protocol |
Refer to the Illumina Gene Expression System Manual
|
| Description |
MIF siRNA(50pmol/ml)-mediated MIF knockdown in HEK293 cells
|
| Data processing |
Cubic spline normalization using Illumina BeadStudio Version "BSGX Version3.2.3"
|
| |
|
| Submission date |
Jan 23, 2008 |
| Last update date |
Mar 29, 2010 |
| Contact name |
Lingfeng Liu |
| E-mail(s) |
liulingfeng1111@gmail.com
|
| Phone |
+86-21-65643262
|
| Organization name |
Fudan University
|
| Department |
Life Science
|
| Lab |
the State Key Laboratory of Genetic Engineering
|
| Street address |
Handan Road 220#
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE10241 |
A Global Genomic View of MIF Knockdown Cell |
|
