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Series GSE102909

Query DataSets for GSE102909

Status

Public on Aug 23, 2017

Title

Peripheral blood transcriptomics following immunization with TLR-adjuvanted nanoparticle vaccine in rhesus macaques

Organism

Experiment type

Expression profiling by array

Summary

Transmission-blocking vaccines (TBVs) aim to disrupt malaria parasite development by targeting antigens expressed in the mosquito midgut and are considered an integral element of malaria eradication efforts. Here, we used non-human primates to demonstrate that immune responses to Pfs25, the leading TBV candidate, can be improved using unconjugated Pfs25 encapsulated in PLGA-based synthetic vaccine particles (SVP[Pfs25]) delivered subcutaneously, compared to the multimeric form, Pfs25-EPA administered intramuscularly. Pfs25-EPA given with the toll-like receptor (TLR)-based adjuvant GLA-LSQ induced robust Ab responses, but SVP[Pfs25] adjuvanted with GLA-LSQ or SVPs containing CpG or R848 increased Ab titers, Pfs25-specific plasmablasts, circulating memory B cells, and plasma cells in the bone marrow. Animals receiving R848 or CpG showed particularly strong type I IFN polarized innate activation, which correlated with increased Ab half-life. Only SVP[Pfs25] induced detectable Pfs25-specific CD4 Th1 and Tfh cells, correlating with improved Ab avidity. Collectively, multiple aspects of the responses elicited to Pfs25 indicate that the nanoparticle technology combined with TLR agonists is a promising platform for TBVs.

Overall design

Four groups of rhesus macaques (n=6/group) were immunized with nanoparticles adjuvanted with nanoparticles containing CpG or R848 or the liposomal adjuvant GLA-LSQ. Whole blood was collected prior to and 24 hours after immunization and gene expression profiles were compared to cellular activation as determined by flow cytometry and cytokine production in plasma.

Contributor(s)

Thompson E, Loré K

Citation missing

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Submission date

Aug 22, 2017

Last update date

Jul 25, 2021

Contact name

Elizabeth Anne Thompson

E-mail(s)

elizabeth.thompson@ki.se

Phone

851776698

Organization name

Karolinska Institutet

Department

Department of Medicine, Solna

Lab

Loré

Street address

L2:04

City

Stockholm

ZIP/Postal code

17176

Country

Sweden

Platforms (1)

Agilent-026806 M. mulatta (Rhesus) Oligo Microarray v2 (Feature Number version)

Samples (44)

More...

GSM2748156	SVP[Pfs25}+SVP[CpG] Pre-bleed [E04_4 prebleed]
GSM2748157	SVP[Pfs25}+SVP[CpG] Pre-bleed [E07_7 prebleed]
GSM2748158	SVP[Pfs25}+SVP[CpG] Pre-bleed [E10_10 prebleed]

GSM2748159	SVP[Pfs25}+SVP[CpG] Pre-bleed [E13_13 prebleed]
GSM2748160	SVP[Pfs25}+SVP[CpG] Pre-bleed [E17_17 prebleed]
GSM2748161	SVP[Pfs25]+SVP[R848] Pre-bleed [E01_1 prebleed]
GSM2748162	SVP[Pfs25]+SVP[R848] Pre-bleed [E02_2 prebleed]
GSM2748163	SVP[Pfs25]+SVP[R848] Pre-bleed [E03_3 prebleed]
GSM2748164	SVP[Pfs25]+SVP[R848] Pre-bleed [E11_11 prebleed]
GSM2748165	SVP[Pfs25]+SVP[R848] Pre-bleed [E16_16 prebleed]
GSM2748166	SVP[Pfs25]+SVP[R848] Pre-bleed [E19_19 prebleed]
GSM2748167	SVP[Pfs25]+GLA-LSQ Pre-bleed [E12_12 prebleed]
GSM2748168	SVP[Pfs25]+GLA-LSQ Pre-bleed [E06_6 prebleed]
GSM2748169	SVP[Pfs25]+GLA-LSQ Pre-bleed [E09_9 prebleed]
GSM2748170	SVP[Pfs25]+GLA-LSQ Pre-bleed [E15_15 prebleed]
GSM2748171	SVP[Pfs25]+GLA-LSQ Pre-bleed [E21_21 prebleed]
GSM2748172	SVP[Pfs25]+GLA-LSQ Pre-bleed [E24_24 prebleed]
GSM2748173	Pfs25-EPA+GLA-LSQ Pre-bleed [E05_5 prebleed]
GSM2748174	Pfs25-EPA+GLA-LSQ Pre-bleed [E08_8 prebleed]
GSM2748175	Pfs25-EPA+GLA-LSQ Pre-bleed [E14_14 prebleed]
GSM2748176	Pfs25-EPA+GLA-LSQ Pre-bleed [E20_20 prebleed]
GSM2748177	Pfs25-EPA+GLA-LSQ Pre-bleed [E22_22 prebleed]
GSM2748178	SVP[Pfs25}+SVP[CpG] 24 hours [E04_28 24h]
GSM2748179	SVP[Pfs25}+SVP[CpG] 24 hours [E07_31 24h]
GSM2748180	SVP[Pfs25}+SVP[CpG] 24 hours [E10_34 24h]
GSM2748181	SVP[Pfs25}+SVP[CpG] 24 hours [E13_37 24h]
GSM2748182	SVP[Pfs25}+SVP[CpG] 24 hours [E17_41 24h]
GSM2748183	SVP[Pfs25]+SVP[R848] 24 hours [E01_25 24h]
GSM2748184	SVP[Pfs25]+SVP[R848] 24 hours [E02_26 24h]
GSM2748185	SVP[Pfs25]+SVP[R848] 24 hours [E03_27 24h]
GSM2748186	SVP[Pfs25]+SVP[R848] 24 hours [E11_35 24h]
GSM2748187	SVP[Pfs25]+SVP[R848] 24 hours [E16_40 24h]
GSM2748188	SVP[Pfs25]+SVP[R848] 24 hours [E19_43 24h]
GSM2748189	SVP[Pfs25]+GLA-LSQ 24 hours [E06_30 24h]
GSM2748190	SVP[Pfs25]+GLA-LSQ 24 hours [E09_33 24h]
GSM2748191	SVP[Pfs25]+GLA-LSQ 24 hours [E12_36 24h]
GSM2748192	SVP[Pfs25]+GLA-LSQ 24 hours [E15_39 24h]
GSM2748193	SVP[Pfs25]+GLA-LSQ 24 hours [E21_45 24h]
GSM2748194	SVP[Pfs25]+GLA-LSQ 24 hours [E24_48 24h]
GSM2748195	Pfs25-EPA+GLA-LSQ 24 hours [E05_29 24h]
GSM2748196	Pfs25-EPA+GLA-LSQ 24 hours [E08_32 24h]
GSM2748197	Pfs25-EPA+GLA-LSQ 24 hours [E14_38 24h]
GSM2748198	Pfs25-EPA+GLA-LSQ 24 hours [E20_44 24h]
GSM2748199	Pfs25-EPA+GLA-LSQ 24 hours [E22_46 24h]

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE102909_RAW.tar	98.0 Mb	(http)(custom)	TAR (of TXT)
Processed data included within Sample table

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