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| Status |
Public on Oct 18, 2017 |
| Title |
Gene expression of Desulfovibrio vulgaris Hildenborough (DvH) strain ES10-5 evolved under salt stress for 5000 generations |
| Organism |
Nitratidesulfovibrio vulgaris str. Hildenborough |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of gene expression changes in a DvH genotype ES10-5, a strain isolated from population ES10 which has been evolved under salt stress for 5000 generations. The gene expression was compared to a gentype ES9-11 isolated from ES9 evolved under the same condition for 1200 generations and the ancestral strain.
The genotype ES10-5 was characterized in this study. ES9-11 was isolated and characterized in Zhou A et al., 2013. Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution. ISME J, 7(9), 1790-1802
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| Overall design |
Basal gene expression changes in salt-stressed evolved genotype 5000g-ES10-5 was examined and compared with salt-stress evolved genotype 1200g-ES9-11 and ancestor at mid-logarithmic phase in standard growth medium LS4D. The transcriptional responses to high salinity in evolved genotypes ES10-5 and ES9-11 and ancestor were characterized by comparing the gene expressions at mid-logarithmic phase under high salinity (LS4D+300 mM NaCl) to standard growth condition LS4D. Total RNA isolated from three replicates of salt-stress evolved 5000g-ES10-5,1200g-ES9-11, or ancestral DvH was labeled with Cy3 and co-hybridized with Cy5-labeled DvH gDNA to the individual array on a custom 12-plex NimbleGen oligonucleotide array.
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| Contributor(s) |
Aifen Z, Jizhong Z |
| Citation(s) |
29138306 |
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| Submission date |
Sep 13, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Aifen Zhou |
| E-mail(s) |
Aifen.Zhou-1@ou.edu
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| Organization name |
University of Oklahoma
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| Department |
Microbiology & Plant Biology
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| Street address |
101 David L. Boren Blvd
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| City |
Norman |
| State/province |
OK |
| ZIP/Postal code |
73019 |
| Country |
USA |
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| Platforms (1) |
| GPL24011 |
NimbleGen design name 120223_DvH_Mm_11-11_JV Desulfovibrio vulgaris str. Hildenborough |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA407069 |
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