|
| Status |
Public on May 21, 2008 |
| Title |
Conservation of small RNA pathways in platypus |
| Organisms |
Ornithorhynchus anatinus; Tachyglossus aculeatus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Small RNA pathways play evolutionarily conserved roles in gene regulation and in defense from pathogenic and parasitic nucleic acids. The character and expression patterns of small RNAs show conservation throughout animal lineages, but specific animal clades also show variations on these recurring themes, including species-specific small RNAs. The monotremes, with only platypus and four species of echidna as extant members, represent the basal branch of the mammalian lineage. Here, we examine the small RNA pathways of monotremes by deep sequencing of six platypus and echidna tissues. We find that highly conserved microRNA species display their signature tissue-specific expression patterns. In addition, we find a large rapidly-evolving cluster of miRNAs on platypus chromosome X1 which is unique to monotremes. Platypus and echidna testes contain a robust piRNA system which appears to be participating in ongoing transposon defense.
Keywords: piRNA
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| Overall design |
Two size fractions of small RNAs (18-24nt and 25-33nt) were cloned and sequenced from platypus adult testis total RNA; one size fraction (28-24nt) was cloned and sequenced from echidna adult testis total RNA
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| Contributor(s) |
Murchison EP, Hannon GJ |
| Citation(s) |
18463306 |
| |
| Submission date |
Feb 19, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
ravi Sachidanandam |
| E-mail(s) |
sachidan@cshl.edu
|
| Phone |
516 367 8864
|
| Fax |
516 367 8389
|
| URL |
http://katahdin.cshl.org:9331
|
| Organization name |
Cold Spring Harbor Laboratory
|
| Department |
N/A
|
| Lab |
sachidanandam
|
| Street address |
1 bungtown road
|
| City |
Cold spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11057 |
Illumina Genome Analyzer (Ornithorhynchus anatinus) |
| GPL11058 |
Illumina Genome Analyzer (Tachyglossus aculeatus) |
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| Samples (3) |
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| Relations |
| SRA |
SRP003805 |
| BioProject |
PRJNA107829 |
