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Series GSE10571 Query DataSets for GSE10571
Status Public on May 21, 2008
Title Conservation of small RNA pathways in platypus
Organisms Ornithorhynchus anatinus; Tachyglossus aculeatus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Small RNA pathways play evolutionarily conserved roles in gene regulation and in defense from pathogenic and parasitic nucleic acids. The character and expression patterns of small RNAs show conservation throughout animal lineages, but specific animal clades also show variations on these recurring themes, including species-specific small RNAs. The monotremes, with only platypus and four species of echidna as extant members, represent the basal branch of the mammalian lineage. Here, we examine the small RNA pathways of monotremes by deep sequencing of six platypus and echidna tissues. We find that highly conserved microRNA species display their signature tissue-specific expression patterns. In addition, we find a large rapidly-evolving cluster of miRNAs on platypus chromosome X1 which is unique to monotremes. Platypus and echidna testes contain a robust piRNA system which appears to be participating in ongoing transposon defense.
Keywords: piRNA
Overall design Two size fractions of small RNAs (18-24nt and 25-33nt) were cloned and sequenced from platypus adult testis total RNA; one size fraction (28-24nt) was cloned and sequenced from echidna adult testis total RNA
Contributor(s) Murchison EP, Hannon GJ
Citation(s) 18463306
Submission date Feb 19, 2008
Last update date May 15, 2019
Contact name ravi Sachidanandam
Phone 516 367 8864
Fax 516 367 8389
Organization name Cold Spring Harbor Laboratory
Department N/A
Lab sachidanandam
Street address 1 bungtown road
City Cold spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
Platforms (2)
GPL11057 Illumina Genome Analyzer (Ornithorhynchus anatinus)
GPL11058 Illumina Genome Analyzer (Tachyglossus aculeatus)
Samples (3)
GSM266831 Platypus testis 18-24 nt
GSM266832 Platypus testis 25-33 nt
GSM266833 Echidna testis 18-24 nt
SRA SRP003805
BioProject PRJNA107829

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