Total RNA extracted using standard Trizol extraction. Total RNA fractionated on 15% polyacrylamide gel and 3' and 5' adaptors sequentially ligated requiring a 3' hydroxyl group and 5' phosphate.
Library strategy
OTHER
Library source
transcriptomic
Library selection
other
Instrument model
Illumina Genome Analyzer
Description
n/a
Data processing
The 3' linker, CTGTAGGCACCATCAAT, was identified and stripped out of the raw reads using a smith-waterman based alignment algorithm.
