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Series GSE105778 Query DataSets for GSE105778
Status Public on Dec 10, 2018
Title Regulation of Glucose Uptake and Inflammation by FOXO1 and FOXO3 in Skeletal Muscle
Organism Mus musculus
Experiment type Expression profiling by array
Summary Forkhead box class O (FoxO) transcription factors regulate whole body energy metabolism, skeletal muscle mass and substrate switching. To elucidate the role of FOXO in skeletal muscle, dominant negative (dn) constructs for FOXO1 (FOXO1dn) or FOXO3 (FOXO3dn) were transfected by electroporation into mouse tibialis anterior muscle and glucose uptake, signal transduction, and glucose stimulated gene expression profiles were assessed. Results were compared against contralateral control transfected muscle.
Transcriptomic analysis revealed major pathways affected by FOXO1dn or FOXO3dn revolve around metabolism and inflammation.
 
Overall design 6 mice for FOXO1dn or FOXO3dn were electroporated in tibialis anterior with either construct or empty vector in contralateral leg. One week post-electroporation, mice were fasted for 4 h, and glucose uptake was measured in vivo using a modified oral glucose tolerance test. Mice were anesthetized with an intraperitoneal injection of Avertin, and electroporated tibialis anterior muscle was removed and snap-frozen. RNA was extracted from electroporated muscle utilizing the EZ RNA extraction kit, and hybridized to an Affymetrix Mouse Gene 2.1 ST array
 
Contributor(s) Lundell LS, Massart J, Krook A, Zierath JR
Citation(s) 30502001
Submission date Oct 23, 2017
Last update date Dec 14, 2018
Contact name Leonidas S Lundell
E-mail(s) leonidas.lundell@gmail.com
Organization name Karolinska Institute
Department Physiology and Pharmacology
Lab Integrative Physiology
Street address Von Eulers Väg 4a
City Stockholm
State/province Stockholm
ZIP/Postal code 171 77
Country Sweden
 
Platforms (1)
GPL17400 [MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version]
Samples (24)
GSM2828106 FOXO1 - FOXO1dn transfection - mouse 1
GSM2828107 FOXO1 - control plasmid transfection - mouse 1
GSM2828108 FOXO1 - FOXO1dn transfection - mouse 2
Relations
BioProject PRJNA415384

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Supplementary file Size Download File type/resource
GSE105778_RAW.tar 110.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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