|
| Status |
Public on Dec 10, 2018 |
| Title |
FOXO1 - FOXO1dn transfection - mouse 4 |
| Sample type |
RNA |
| |
|
| Source name |
Tibialis anterior, 2 hours post oral glucose tolerance test, FOXOdn plasmid
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57B6/J
age: 30 weeks
tissue: Tibialis anterior
|
| Treatment protocol |
Male, 30 weeks, C57BL/6J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
EZ RNA extraction kit according to manufacturer instruction
|
| Label |
biotin
|
| Label protocol |
Sense strand cDNA was synthesized from total RNA with Ambion® WT Expression Kit (Life Techniologies) and biotin labeled with the Affymetrix® GeneChip WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridized to Affymetrix Human Mouse Gene ST Array 2.1 according to manufacturer's protocols in a GeneChip Hybridization Oven 640 (Affymetrix). Arrays were washed and stained with a GeneChip Gluidics station 450 (Affymetrix).
|
| Scan protocol |
Arrays were scanned with a GeneChip Scanner 3000 7G (Affymetrix).
|
| Data processing |
Pre-processing of data was performed using Robust Multi-array Average (RMA) using oligo, mogene21sttranscriptcluster.db, and collapsed using WGCNA.
|
| |
|
| Submission date |
Oct 23, 2017 |
| Last update date |
Dec 11, 2018 |
| Contact name |
Leonidas S Lundell |
| E-mail(s) |
leonidas.lundell@gmail.com
|
| Organization name |
Karolinska Institute
|
| Department |
Physiology and Pharmacology
|
| Lab |
Integrative Physiology
|
| Street address |
Von Eulers Väg 4a
|
| City |
Stockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
171 77 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE105778 |
Regulation of Glucose Uptake and Inflammation by FOXO1 and FOXO3 in Skeletal Muscle |
|
