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| Status |
Public on Mar 01, 2018 |
| Title |
The effect of MAC3 and MAC5A on small RNA accumulation |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MAC5A and MAC3 are conserved proteins that are required for development and immunity. They promotes the accumulation of microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs), which repress gene expression. In this project, we aim to determine the global effect of MAC3 and MAC5Aon the accumulation of miRNAs and siRNAs. We compared the small RNA profile in mac3a mac3b and mac5a with that in Col (Wild-type, WT). Small RNA libraries prepared from inflorescences of mac3a mac3b, mac5a and Col was subjected to Illumina deep sequencing analyses. The results show that many miRNAs and siRNAs were reduced in abundance in mac5a and mac3a mac3b relative to Col in two biological replicates.
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| Overall design |
non coding RNA deep sequencing
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| |
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| Contributor(s) |
Shengjun L, Bin Y, Chi Z |
| Citation missing |
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| |
| Submission date |
Oct 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Chi Zhang |
| E-mail(s) |
czhang5@unl.edu
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| Organization name |
University of Nebraska - Lincoln
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| Street address |
1901 Vine Street
|
| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588 |
| Country |
USA |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA415623 |
| SRA |
SRP121328 |
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