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Series GSE106110 Query DataSets for GSE106110
Status Public on Mar 01, 2018
Title The effect of MAC3 and MAC5A on small RNA accumulation
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary MAC5A and MAC3 are conserved proteins that are required for development and immunity. They promotes the accumulation of microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs), which repress gene expression. In this project, we aim to determine the global effect of MAC3 and MAC5Aon the accumulation of miRNAs and siRNAs. We compared the small RNA profile in mac3a mac3b and mac5a with that in Col (Wild-type, WT). Small RNA libraries prepared from inflorescences of mac3a mac3b, mac5a and Col was subjected to Illumina deep sequencing analyses. The results show that many miRNAs and siRNAs were reduced in abundance in mac5a and mac3a mac3b relative to Col in two biological replicates.
Overall design non coding RNA deep sequencing
Contributor(s) Shengjun L, Bin Y, Chi Z
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Submission date Oct 24, 2017
Last update date May 15, 2019
Contact name Chi Zhang
Organization name University of Nebraska - Lincoln
Street address 1901 Vine Street
City Lincoln
State/province NE
ZIP/Postal code 68588
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (6)
GSM2829820 WT Rep1
GSM2829821 WT Rep2
GSM2829822 3ab Rep 1
BioProject PRJNA415623
SRA SRP121328

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Supplementary file Size Download File type/resource
GSE106110_RAW.tar 20.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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