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| Status |
Public on Dec 31, 2017 |
| Title |
Transcriptomic response to adult hypothalamic deletion of Snord116 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Snord116 deletion mouse models recapitulate aspects of the Prader-Willi Syndrome. In this study, we examine the gene expression changes in the mediobasal hypothalamus for mice which have an adult onset deletion of Snord116 in the mediobasal hypothalamus.
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| Overall design |
Snord116 floxed mice were stereotaxically injected in the mediobasal hypothalamus with adeno-associated viruses (AAVs) expressing Cre or GFP. RNA sequencing was performed on laser-captured hypothalamic regions where Cre was expressed as well as in matching regions from GFP injected controls. AAV-Cre treated animals were also classed based on their body weight phenotype as obese or non-obese.
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| Contributor(s) |
Polex-Wolf J, Lam BY, Coll AP, O'Rahilly S, Yeo GS |
| Citation(s) |
29376887 |
| |
| Submission date |
Oct 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA415925 |
| SRA |
SRP121633 |
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