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| Status |
Public on Dec 25, 2017 |
| Title |
Immune response induced by an HSV-1 mutant strain: a discrepancy analysis of immune system gene profile in comparison with a wild type virus strain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We previously generated an HSV-1 mutant strain, M3, which contained partial deletions in ul7, ul41 and LAT and produced an attenuated phenotype in mice. In the present study, we performed a comparative analysis to characterize the immune responses induced by M3 versus wild type HSV-1 in a mouse model. Infection with wild type HSV-1 triggered an inflammatory-dominated response and the suppression of adaptive immunity and was accompanied by severe pathological damage. In contrast, infection with M3 induced a systematic immune response involving the full activation of both innate and adaptive immunity and was accompanied by no obvious pathological changes.
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| Overall design |
The mRNA expression profile in splenocytes of HSV-1 mutant strain M3 infected (3×10^4 PFU) and HSV-1 wild type strain Mckrae infected (3×10^4 PFU) BALB/c mice were measured at day 7 post-infection, and uninfected mice were used as control. The mRNA expression profile in splenocytes of HSV-1 mutant strain M3 infected (3×10^4 PFU) mice were also measured at day 30 and day 60 post-infection. Three independent experiments were performed at each time using different mice for each experiment.
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| Contributor(s) |
Zhang X, Li Q |
| Citation missing |
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| Submission date |
Dec 24, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Xiaolong Zhang |
| Organization name |
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
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| Street address |
935 jiaoling Road
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| City |
Kunming |
| ZIP/Postal code |
650118 |
| Country |
China |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA427435 |
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