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| Status |
Public on Mar 09, 2018 |
| Title |
MalR regulon of B. breve UCC2003; malto-oligosachrides and beyond |
| Organism |
Bifidobacterium breve UCC2003 |
| Experiment type |
Expression profiling by array
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| Summary |
Bifidobacteria resident in the gastrointestinal tract are subject to many stresses such as bile stress, osmotic stress and starvation. Adaption to these stresses requires a high amount of energy and rapid changes in gene transcription. Four Bifidobacterium breve UCC2003-encoded Lac I type transcriptional regulators had been proposed to be involved in the utilisation of maltose, maltodextrins and related polymers such as starch, amylopectin, amylose, glycogen and pullulan. However, it now appears that these regulators are also involved in the utilisation of other carbon sources such as ribose and cellobiose. Interestingly, in vitro these regulators often cross regulate the same carbohydrate utilization genes, along with regulating each other. This hierarchical regulatory system controls the transcription of genes involved in carbohydrate uptake, storage, breakdown and central metabolism. These four regulators respond to differing effectors in vitro, these effectors include sugars such as turanose or galactose, thus indicating that each regulator is responsible for a different aspect of carbon metabolism. This complex network of gene regulation provide novel insights into the decision making process of the cell and the metabolic adaption of bifidobacteria to its environment.
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| Overall design |
DNA-microarrays containing oligonucleotide primers representing each of the 1864 annotated genes on the genome of B. breve UCC2003 (O'Connell Motherway et al., 2011) were designed by and obtained from Agilent Technologies (Palo Alto, Ca., USA). Methods for cell disruption, RNA isolation, RNA quality control, complementary DNA synthesis and labeling were performed as described previously (Pokusaeva et al., 2009). Labeled cDNA was hybridized using the Agilent Gene Expression hybridization kit (part number 5188-5242) as described in the Agilent Two-Color Microarray-Based Gene Expression Analysis v4.0 manual (G4140-90050). Following hybridization, microarrays were washed in accordance with Agilent’s standard procedures and scanned using an Agilent DNA microarray scanner (model G2565A). Generated scans were converted to data files with Agilent's Feature Extraction software (Version 9.5). DNA-microarray data were processed as previously described (Garcia De La Nava et al., 2003). Differential expression tests were performed with the Cyber-T implementation of a variant of the t-test (Long et al., 2001). A gene was considered differentially expressed when p < 0.001 and an expression ratio of >3 or <0.33 relative to the control.
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| Contributor(s) |
Lanigan N, Kelly E, van Sinderen D |
| Citation missing |
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| Submission date |
Jan 09, 2018 |
| Last update date |
Mar 10, 2018 |
| Contact name |
Noreen Lanigan |
| E-mail(s) |
n.lanigan@umail.ucc.ie
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| Organization name |
University college cork
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| Department |
Microbiology
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| Lab |
5.27
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| Street address |
Biosciences Institute, University college Cork, cork, ireland.
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| City |
Cork |
| State/province |
Cork |
| ZIP/Postal code |
- |
| Country |
Ireland |
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| Platforms (1) |
| GPL13210 |
Agilent-020573 Bifidobacterium breve UCC2003 Agilent 4x44k format |
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| Samples (8)
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| GSM2916423 |
B. breve UCC2003 v. B. breve UCC2003-malR2 |
| GSM2916424 |
B. breve UCC2003-malR2 v. B. breve UCC2003 |
| GSM2916425 |
B. breve UCC2003 v. B. breve UCC2003-malR3 |
| GSM2916426 |
B. breve UCC2003-malR3 v. B. breve UCC2003 |
| GSM2916427 |
B. breve UCC2003 v. B. breve UCC2003-malR5 |
| GSM2916428 |
B. breve UCC2003-malR5 v. B. breve UCC2003 |
| GSM2916429 |
B. breve UCC2003 v. B. breve UCC2003-malR6 |
| GSM2916430 |
B. breve UCC2003-malR6 v. B. breve UCC2003 |
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| Relations |
| BioProject |
PRJNA429225 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE108950_RAW.tar |
68.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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