|
| Status |
Public on Dec 22, 2008 |
| Title |
Gene expression profiling of liver metastases from colorectal cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
At present, medical treatments of synchronous and metachronous liver metastases from colorectal cancer are not differentiated. The aim of the study was to analyze the gene expression profiling of synchronous and metachronous lesions in order to identify molecular signatures as possible basis for choice of systemic therapies. Fresh tissues specimens from metastases of 18 patients undergone liver surgery were collected (10 synchronous and 8 metachronous lesions). Gene expression profiling was studied using Affymetrix platform. Two different profiles were identified. Pathway related to the Epidermal Growth Factor receptor (EGFr) was upregulated in metachronous lesions whereas pathways mainly related to inflammation in synchronous lesions. Real Time-PCR, Western Blotting and ELISA confirmed that the metachronous lesions had the overexpression of EGFr, but the synchronous ones had the overexpression of Cyclo-oxygenase 2 (COX-2). These results suggest that synchronous or metachronous liver metastases from colorectal cancer could be differently treated on the basis of different molecular pathways.
Keywords: disease state analysis
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| Overall design |
Gene expression analysis of liver metastases from 18 colorectal cancer patients: 8 metachronous and 10 synchronous single metastatic lesions.
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| |
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| Contributor(s) |
Pantaleo MA, Astolfi A, Nannini M, Paterini P, Piazzi G, Ercolani G, Brandi G, Andrea P, Pinna AD, Biasco G |
| Citation(s) |
18827815 |
| Submission date |
Mar 27, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Maria Abbondanza Pantaleo |
| E-mail(s) |
maria.pantaleo@unibo.it
|
| Organization name |
Policlinico S. Orsola-Malpighi, University of Bologna
|
| Department |
Institute of Hematology and Medical Oncology "L.A.Seragnoli"
|
| Street address |
via Massarenti 9
|
| City |
Bologna |
| State/province |
BO |
| ZIP/Postal code |
40138 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (18)
|
| GSM277231 |
Metachronous liver metastasis colorectal CA 02 |
| GSM277236 |
Metachronous liver metastasis colorectal CA 03 |
| GSM277238 |
Metachronous liver metastasis colorectal CA 08 |
| GSM277239 |
Metachronous liver metastasis colorectal CA 09 |
| GSM277246 |
Metachronous liver metastasis colorectal CA 22 |
| GSM277248 |
Metachronous liver metastasis colorectal CA 23 |
| GSM277253 |
Metachronous liver metastasis colorectal CA 24 |
| GSM277256 |
Metachronous liver metastasis colorectal CA 28 |
| GSM277466 |
Synchronous liver metastasis colorectal CA 05 |
| GSM277469 |
Synchronous liver metastasis colorectal CA 06 |
| GSM277477 |
Synchronous liver metastasis colorectal CA 07 |
| GSM277478 |
Synchronous liver metastasis colorectal CA 12 |
| GSM277479 |
Synchronous liver metastasis colorectal CA 13 |
| GSM277481 |
Synchronous liver metastasis colorectal CA 14 |
| GSM277494 |
Synchronous liver metastasis colorectal CA 15 |
| GSM277646 |
Synchronous liver metastasis colorectal CA 18 |
| GSM277647 |
Synchronous liver metastasis colorectal CA 17 |
| GSM277648 |
Synchronous liver metastasis colorectal CA 19 |
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| Relations |
| BioProject |
PRJNA107161 |
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