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Status |
Public on Jun 14, 2018 |
Title |
The fate of CD8+ T cells during infection is linked to their developmental origin [Virtual_Memory_following_infection_timestamp] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
During immune ontogeny the thymus is colonized by distinct waves of hematopoietic stem cells that give rise to unique lineages of immune cells. In this report, we asked whether the developmental origin of CD8+ T cells influences their response to infection later in adulthood. To answer this question, we developed a system to ‘timestamp’ CD8+ T cells in situ at various stages of development (1d and 28d) and examined their behavior after thymic differentiation. We found that neonatal-derived CD8+ T cells have an intrinsic propensity to become virtual memory cells prior to infection and are the first cells to proliferate and become effectors after microbial challenge. These data indicate that there are developmental layers in the adult CD8+ T cell response to infection and that the heterogeneity in the effector pool is linked to the variation in the developmental origins of the responding cells. This dataset profiles gene expression in 1day- and 28day-timestamped virtual memory (CD44hi) CD8+ T cells aged for 4 weeks of post-thymic differentiation and then transferred into congenically marked recipients. Timestamped cells were recovered 5 days following infection with Listeria monocytogenes.
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Overall design |
Gene expression profiling of virtual memory (CD44hi) transgenic CD8+ T cells, 5 days following infection with Listeria, comparing cells made at 1 day or 28 days of life (in duplicate).
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Contributor(s) |
Smith N, Rudd B, Grenier J |
Citation(s) |
29909981 |
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Submission date |
Jan 28, 2018 |
Last update date |
Mar 25, 2019 |
Contact name |
Jennifer K Grenier |
Organization name |
Cornell University
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Department |
Biomedical Sciences
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Lab |
Biotechnology Building rm 333
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Street address |
526 Campus Rd
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City |
Ithaca |
State/province |
NY |
ZIP/Postal code |
14853 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (4)
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GSM2966342 |
VM CD8+ cells timestamped at day 1, moved to congenic recipients at 4 wks, collected 5 dpi (Listeria), rep 1 |
GSM2966343 |
VM CD8+ cells timestamped at day 1, moved to congenic recipients at 4 wks, collected 5 dpi (Listeria), rep 2 |
GSM2966344 |
VM CD8+ cells timestamped at day 28, moved to congenic recipients at 8 wks, collected 5 dpi (Listeria), rep 1 |
GSM2966345 |
VM CD8+ cells timestamped at day 28, moved to congenic recipients at 8 wks, collected 5 dpi (Listeria), rep 2 |
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This SubSeries is part of SuperSeries: |
GSE97802 |
The fate of CD8+ T cells during infection is linked to their developmental origin |
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Relations |
BioProject |
PRJNA431923 |
SRA |
SRP131625 |