|
| Status |
Public on Mar 31, 2018 |
| Title |
Iron starvation in Bifidobacterium breve UCC2003 increases bile resistance through enhanced expression of a bile salt hydrolase |
| Organism |
Bifidobacterium breve UCC2003 |
| Experiment type |
Expression profiling by array
|
| Summary |
Bifidobacterium are considered to be beneficial for human health and are classified as probiotic bacterium. They must resist many environmental stress factors in order to survive in the gastrointestinal environment including; pH, oxygen availability, bile and nutrient starvation (eg: iron or carbon). This study investigates Bifidobacterium breve UCC2003 global genome response to growth under ferrous and/or ferric iron limiting conditions. Revealing that growth under iron limitation effects many processes in the cell including carbon and nitrogen metabolism and induces/reduces the expression of numerous genes; including multiple iron uptake systems, DPS proteins (which are predicted to be involved in iron storage/DNA protection), Fe-S cluster associated proteins and a bile salt hydrolase (bshB). Insertional mutagenesis and survival assays were employed and demonstrated that iron starvation imposed on B. breve UCC2003 results in an increased resistance to bile stress due to in part the iron-inducible transcription of the bshB gene. Furthermore, this study links BSH activity in B. breve UCC2003 to its ability to survive the deleterious effects of bile salt and suggest that B. breve UCC2003 may be use iron as a signal to adapt to the constantly changing environment within the small intestine.
|
| |
|
| Overall design |
DNA-microarrays containing oligonucleotide primers representing each of the 1864 annotated genes on the genome of B. breve UCC2003 (O'Connell Motherway et al., 2011) were designed by and obtained from Agilent Technologies (Palo Alto, Ca., USA). Methods for cell disruption, RNA isolation, RNA quality control, complementary DNA synthesis and labeling were performed as described previously (Pokusaeva et al., 2009). Labeled cDNA was hybridized using the Agilent Gene Expression hybridization kit (part number 5188-5242) as described in the Agilent Two-Color Microarray-Based Gene Expression Analysis v4.0 manual (G4140-90050). Following hybridization, microarrays were washed in accordance with Agilent’s standard procedures and scanned using an Agilent DNA microarray scanner (model G2565A). Generated scans were converted to data files with Agilent's Feature Extraction software (Version 9.5). DNA-microarray data were processed as previously described (Garcia De La Nava et al., 2003). Differential expression tests were performed with the Cyber-T implementation of a variant of the t-test (Long et al., 2001). A gene was considered differentially expressed when p < 0.001 and an expression ratio of >3 or <0.33 relative to the control.
|
| |
|
| Contributor(s) |
Lanigan N, van Sinderen D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 02, 2018 |
| Last update date |
Apr 01, 2018 |
| Contact name |
Noreen Lanigan |
| E-mail(s) |
n.lanigan@umail.ucc.ie
|
| Organization name |
University college cork
|
| Department |
Microbiology
|
| Lab |
5.27
|
| Street address |
Biosciences Institute, University college Cork, cork, ireland.
|
| City |
Cork |
| State/province |
Cork |
| ZIP/Postal code |
- |
| Country |
Ireland |
| |
|
| Platforms (2) |
| GPL13210 |
Agilent-020573 Bifidobacterium breve UCC2003 Agilent 4x44k format |
| GPL24137 |
Agilent-015669 Bifidobacterium breve UCC2003 array (Feature Number version) |
|
| Samples (5)
|
| GSM2977340 |
F-RCM v. F-RCM and stressed with 85µM ciclopirox olamine |
| GSM2977341 |
F-RCM and stressed with 85µM ciclopirox olamine vs F-RCM |
| GSM2977342 |
F-RCM v. F-RCM and stressed with 100µM phenanthroline |
| GSM2977343 |
F-RCM and stressed with 100µM phenanthroline vs F-RCM |
| GSM2977344 |
F-RCM v. F-RCM and stressed with 275µM Dipyridyl |
|
| Relations |
| BioProject |
PRJNA432734 |
Less...
More...