|
| Status |
Public on Feb 28, 2018 |
| Title |
Single-cell transcriptome analyses reveal endothelial cell heterogeneity in tumors and changes following anti-angiogenic treatment |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand tumor stromal cell heterogeneity focusing on tumor endothelial cells and tumor-associated fibroblasts, we isolated single cells from COLO205 tumor xenograft grown sub-cutaneously in SCID mice. To enrich stromal cell content, we removed vast majority of tumor cells by a depletion protocol using anti-CD24 and anti-E-Cadherin antibodies. The remaining single cells were profiled by single cell RNAseq
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| |
|
| Overall design |
Tumors were dissociated, sorted into single cells, and profiled by 1 chromium. Single cell 3' library, Gelbeads and multiplex kit were made by using 10x Genomics platform.
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| |
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| Contributor(s) |
Thurston G |
| Citation(s) |
29449267 |
| |
| Submission date |
Feb 12, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Qi Zhao |
| E-mail(s) |
qi.zhao@regeneron.com
|
| Organization name |
Regeneron Pharmaceuticals Inc
|
| Street address |
777 Old Saw Mill River Road
|
| City |
Tarrytown |
| ZIP/Postal code |
10591 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (16)
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| Relations |
| BioProject |
PRJNA433865 |
| SRA |
SRP132721 |
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