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| Status |
Public on Feb 14, 2018 |
| Title |
Peritoneal cavity macrophage response to IFNg and IFNa |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We seeked to determine in vivo effects of IFNg and IFNa response in peritoneal cavity macrophages. These cells were part of ImmGen Interferon cytokine study and immunocytes were sorted according to Immgen's standard lineage panel. Profiles from peritoneal cavity macrophages were used to analyze cell specific responses to IFNg.
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| Overall design |
8,000 units of recombinant IFNg or 10,000 units of recombinant IFNa were injected subcutaneously in C57bl6 6-week-old male mice. Mice were sacrificed 2 hours after injection and peritoneal lavage was collected. F4/80+ ICAM+ peritoneal cavity macrophages were double sorted using FACSAria into Trizol.
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| Contributor(s) |
Panduro M, Ortiz-Lopez A, Rhoads A, Moodley D, Mathis D, Benoist C |
| Citation(s) |
26824662 |
| |
| Submission date |
Feb 13, 2018 |
| Last update date |
Mar 04, 2019 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
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| Phone |
617-432-7747
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| Organization name |
Harvard Medical School
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| Department |
Microbiology and Immunobiology
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| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA433974 |
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