|
| Status |
Public on Feb 14, 2018 |
| Title |
MF.IFNa.2hr.PC_4 |
| Sample type |
RNA |
| |
|
| Source name |
Peritoneal Cavity
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6
Sex: Male
age: 6 Weeks
treatment: 10000 U IFNa
cell type: Peritoneal cavity macrophages
phenotype markers: F4/80+ ICAM2+
separation method: FACSAria
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip WT Terminal Labeling and Controls Kit.
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Gene 1.0 ST Array.
|
| Scan protocol |
Affymetrix GeneChip Scanner.
|
| Description |
MF.IFNa.2hr.PC#4
|
| Data processing |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Feb 13, 2018 |
| Last update date |
Feb 14, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE75306 |
ImmGen Cytokines: Interferons |
| GSE110549 |
Peritoneal cavity macrophage response to IFNg and IFNa |
|
