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| Status |
Public on Feb 14, 2018 |
| Title |
Zfp36l1 overexpression/knockdown effect on primary mouse articular chondrocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression profiling of primary mouse articular chondrocyte infected with recombinant adenovirus expressing mouse ZFP36 Ring Finger Protein Like 1(Zfp36l1) or recombinant adenovirus expressing mouse small hairpin RNA of Zfp36l1.
In this study, we have attempted to explore the effects of Zfp36l1 gene overexpression or knockdown on mouse transcriptome and have identified numerous genes which are involved in osteoarthritis pathogenesis.
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| Overall design |
Primary mouse articular chondrocytes were isolated from mouse femoral condyles and tibial plateaus cartilage tissue and were infected with null (control) or recombinant adenovirus expressing ZFP36 Ring Finger Protein Like 1 (Ad-Zfp36l1) or small hairpin RNA of Zfp36l1(Ad-shZfp36l1) for 36 hours. At 36 hours post-infection, total RNA was extracted from infected with null control adenovirus (3 biological replicates) or infected with recombinant Ad-Zfp36l1 (3 biological replicates) or recombinant Ad-shZfp36l1 (3 biological replicates) on primary mouse articular chondrocytes and subjected to microarray analysis.
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| Contributor(s) |
Chun J |
| Citation(s) |
30622281 |
| |
| Submission date |
Feb 13, 2018 |
| Last update date |
Jan 15, 2019 |
| Contact name |
Yoonkyung Won |
| E-mail(s) |
ykwon@gist.ac.kr
|
| Organization name |
Gwangju Institute of Science and Technology
|
| Street address |
123 Cheomdangwagiro
|
| City |
Gwangju |
| ZIP/Postal code |
61005 |
| Country |
South Korea |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM3003526 |
Null Control Adenovirus infected primary mouse articular chondrocytes, biological rep 1 |
| GSM3003527 |
Null Control Adenovirus infected primary mouse articular chondrocytes, biological rep 2 |
| GSM3003528 |
Null Control Adenovirus infected primary mouse articular chondrocytes, biological rep 3 |
| GSM3003529 |
Zfp36l1 Adenovirus infected primary mouse articular chondrocytes, biological rep 1 |
| GSM3003530 |
Zfp36l1 Adenovirus infected primary mouse articular chondrocytes, biological rep 2 |
| GSM3003531 |
Zfp36l1 Adenovirus infected primary mouse articular chondrocytes, biological rep 3 |
| GSM3003532 |
Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes, biological rep 1 |
| GSM3003533 |
Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes, biological rep 2 |
| GSM3003534 |
Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes, biological rep 3 |
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| Relations |
| BioProject |
PRJNA434016 |
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