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| Status |
Public on Apr 11, 2018 |
| Title |
Expression analysis of Escherichia coli CC72 before and after osmotic stress |
| Platform organism |
Escherichia coli K-12 |
| Sample organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by array
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| Summary |
Expression level of whole genome genes in Escherichia coli CC72 at early-exponential phase, and 10 min, 20 min and 45 min after osmotic stress.
Venus was fused to the 3' end of rpoC in E. coli MG1655 to localize RNA polymerase as reported previously (C. Cagliero and D. J. Jin, 2013).
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| Overall design |
8 chips were used to measure total RNA extracted from E. coli CC72 grown in LB medium at eary-exponential phase, and 10 min, 20 min and 45 min after 5 M NaCl stress. Each condition has two biological replicates. And each chip measures the expression level of 4252 genes from E. coli MG 1655 eight 60 mers probe pairs (PM/MM) per gene, with two technical replicates.
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| Contributor(s) |
Sun Z, Jin DJ |
| Citation(s) |
30843055 |
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| Submission date |
Feb 16, 2018 |
| Last update date |
Mar 11, 2019 |
| Contact name |
Zhe Sun |
| Organization name |
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
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| Street address |
32 West 7th Ave
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| City |
Tianjin |
| ZIP/Postal code |
300308 |
| Country |
China |
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| Platforms (1) |
| GPL14649 |
NimbleGen E. coli K12 Gene Expression Array [071112_Ecoli_K12_EXP] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA434430 |
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