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| Status |
Public on Dec 14, 2018 |
| Title |
Gene expression Analysis of wild type (WT) and Blnc1 adipose specific transgenic mice (Tg) epididymal WAT (eWAT) Transcriptomes after 21 weeks high fat diet (HFD) feeding |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Long noncoding RNAs (lncRNAs) are emerging as powerful regulators of adipocyte differentiation and gene expression. However, their physiological role in adipose tissue biology and systemic energy metabolism has not been established. Here we show that adipose tissue expression of Blnc1, a conserved lncRNA regulator of thermogenic genes, is highly induced in obese mice. Fat-specific inactivation of Blnc1 impairs cold-induced thermogenesis and browning, exacerbates obesity-associated brown fat whitening, and worsens adipose tissue inflammation and fibrosis, leading to more severe insulin resistance and hepatic steatosis. On the contrary, transgenic expression of Blnc1 in adipose tissue elicits the opposite and beneficial metabolic effects, supporting a critical role of Blnc1 in driving adipose adaptation during obesity. Mechanistically, Blnc1 cell-autonomously attenuates proinflammatory cytokine signaling and promotes fuel storage in adipocytes through its protein partner Zbtb7b. This study illustrates a surprisingly pleiotropic and dominant role of lncRNA in driving adaptive adipose tissue remodeling and preserving metabolic health.
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| Overall design |
eWAT mRNA profiles of WT and Blnc1 adipose specific Tg mice after 21 weeks HFD feeding were generated by microarray, in triplicate
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| Contributor(s) |
Zhao X, Lin J |
| Citation(s) |
29934059 |
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| Submission date |
Mar 15, 2018 |
| Last update date |
Dec 14, 2018 |
| Contact name |
xuyun zhao |
| E-mail(s) |
zhaobig_8888@hotmail.com
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| Organization name |
Life Sciences Institute
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| Street address |
210 washtenaw ave
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| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM3042961 |
Blnc1 adipose specific Tg biological rep1 |
| GSM3042962 |
Blnc1 adipose specific Tg biological rep2 |
| GSM3042963 |
Blnc1 adipose specific Tg biological rep3 |
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| Relations |
| BioProject |
PRJNA438438 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE111865_RAW.tar |
25.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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