|
| Status |
Public on Dec 14, 2018 |
| Title |
Blnc1 adipose specific Tg biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Blnc1 adipose specific Tg, eWAT
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 8 months
gender: male
tissue: eWAT
genotype: Tg
treatment: HFD
|
| Treatment protocol |
WT and Tg mice was fed on HFD diet (D12492, Research Diets) for 21 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mouse eWAT was extracted, immediately frozen in liquid nitrogen, and RNA was harvested using Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2) from 400 ng total RNA
|
| |
|
| Hybridization protocol |
Following labeling procedure, 2.53 ug of cDNA were hybridized at 48C on Mouse ST 2.1, and were washed and stained using the Affymetrix GeneTitan system (software version 3.2.4.1515)
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneTitan system (software version 3.2.4.1515).
|
| Description |
Gene expression data from Tg mice biological rep3
|
| Data processing |
Data was analyzed using the oligo and limma Bioconductor package implemented in the R-statistical language version 3.1.1. RMA was used to fit log2 transformed expression values.
|
| |
|
| Submission date |
Mar 15, 2018 |
| Last update date |
Dec 14, 2018 |
| Contact name |
xuyun zhao |
| E-mail(s) |
zhaobig_8888@hotmail.com
|
| Organization name |
Life Sciences Institute
|
| Street address |
210 washtenaw ave
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE111865 |
Gene expression Analysis of wild type (WT) and Blnc1 adipose specific transgenic mice (Tg) epididymal WAT (eWAT) Transcriptomes after 21 weeks high fat diet (HFD) feeding |
|
