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| Status |
Public on Mar 29, 2018 |
| Title |
In vivo transcriptome analysis of avian pathogenic Escherichia coli under the control of RstA/RstB two-component regulatory system |
| Platform organisms |
Escherichia coli O157:H7 str. EDL933; Escherichia coli CFT073; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655 |
| Sample organism |
Escherichia coli APEC O2 |
| Experiment type |
Expression profiling by array
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| Summary |
APEC cause avian colibacillosis in poultry, characterized by the systematic infection, such as septicemia, airsacculitis, and pericarditis. APEC mainly use two-component regulatory systems (TCSs) to deal with the stressing environments in host during their infection. Whereas most TCSs in E.coli are well characterized, the characterization of RstA/RstB in APEC has not been thoroughly investigated. To understand the whole picture of RstA/RstB regulation, especially its role in virulence regulation, transcriptional analysis of the effect of rstAB deletion was performed in vivo. We compared the transcriptome of rstAB mutant and its wild-type strain during their growth in bloodstream of challenged chickens. In total, the transcripts of 85 genes were down-regulated by rstAB deletion while 113 were up-regulated at least twofold (cutoff limitation for fold change >2 or <0.5 and Cuffdiff P-value <0.05 was used to select differential expression genes). Our data showed that the RstA/RstB was a by-function regulator system, acting as both an activator and a repressor. The RstAB mainly regulated systems involved in nitrogen metabolism, bacterial virulence, iron acquisition and acid resistance.
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| Overall design |
We contructed the rstAB mutant and compared the transcriptome of rstAB mutant and its wild-type strain during their growth in bloodstream of challenged chickens
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| Contributor(s) |
Gao Q, Gao S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 28, 2018 |
| Last update date |
Mar 30, 2018 |
| Contact name |
Qingqing Gao |
| E-mail(s) |
gq00888@sina.com
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| Organization name |
Yangzhou university
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| Department |
Colledge of Veterianry medicine
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| Street address |
Yangzhou wenhui Road 12
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| City |
Yangzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
225009 |
| Country |
China |
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| Platforms (1) |
| GPL13359 |
Agilent-020097 E. coli Gene Expression Microarray (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA445970 |
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