 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 09, 2018 |
| Title |
Role and function of Class III LitR, a photosensor homolog from Burkholderia multivorans ATCC 17616 in the regulation of folate production. |
| Platform organism |
Burkholderia multivorans ATCC 17616 |
| Sample organism |
Burkholderia multivorans |
| Experiment type |
Expression profiling by array
|
| Summary |
The LitR/CarH protein family is a adenosyl B12 (AdoB12)-dependent photoreceptor with DNA-binding activity and its homologs are widely distributed in the genome of diverged bacterial genera. Here, we studied a role and function of a LitR homolog from a Gram-negative soil bacteria Burkholderia multivorans, which has no AdoB12-binding domain. Transcriptome analysis revealed the existence of 19 light-induced genes (folE, cfa, litS, a Photolyase gene phrB2, cryB2) located in the flanking region of litR. Gene disruption of litR caused a constitutive expression of all light-inducible genes, while the mutation of the light-dependent expressed sigma factor litS abolished the transcription in cryB2, phrB2 operon and cfa operon, indicating that LitR and LitS play a central role in the light-inducible transcription. Gel-shift assay showed that a recombinant protein LitR specifically binds to the promoter region of litR and folE2 operon, and its ability was lowered by UV-A illumination. LitR absorbs maximally near 340 nm, and exhibited a photocyclic response and light-dependent subunit dissociation. Furthermore, the litR mutant produced a 20-fold high intracellular folate compared to the wild-type strain. Overall, the line of evidence suggest that Class III LitR light-dependently regulates the transcription of litR itself and folE2-litS operon, leading the production of folate, and then expressed RNA polymerase-LitS directs the transcription for phrB2 operon and cfa operon. Light-dependent characteristics suggests that Class III LitR in complex with a UV-A absorbing molecule has a novel light-sensing mechanism.
|
| |
|
| Overall design |
Chromosomal RNA maps of wild-type cells cultured under the light conditions were compared with those of wild-type cells cultured under the dark conditions
|
| |
|
| Contributor(s) |
Ueda K, Takano H |
| Citation(s) |
30249707 |
| |
| Submission date |
Mar 28, 2018 |
| Last update date |
Oct 11, 2018 |
| Contact name |
Hideaki Takano |
| E-mail(s) |
takano.hideaki@nihon-u.ac.jp
|
| Phone |
81466843936
|
| Organization name |
Nihon University
|
| Department |
College of Bioresource Sciences
|
| Street address |
1866, Kameino
|
| City |
Fujisawa |
| State/province |
KANAGAWA |
| ZIP/Postal code |
2520880 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL24783 |
NimbleGen-Burkholderia multivorans ATCC 17616 385K 4-plex custom array [110225_RDKK301_Bmul_expr] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA447436 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112472_RAW.tar |
10.8 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...