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Series GSE112472 Query DataSets for GSE112472
Status Public on Oct 09, 2018
Title Role and function of Class III LitR, a photosensor homolog from Burkholderia multivorans ATCC 17616 in the regulation of folate production.
Platform organism Burkholderia multivorans ATCC 17616
Sample organism Burkholderia multivorans
Experiment type Expression profiling by array
Summary The LitR/CarH protein family is a adenosyl B12 (AdoB12)-dependent photoreceptor with DNA-binding activity and its homologs are widely distributed in the genome of diverged bacterial genera. Here, we studied a role and function of a LitR homolog from a Gram-negative soil bacteria Burkholderia multivorans, which has no AdoB12-binding domain. Transcriptome analysis revealed the existence of 19 light-induced genes (folE, cfa, litS, a Photolyase gene phrB2, cryB2) located in the flanking region of litR. Gene disruption of litR caused a constitutive expression of all light-inducible genes, while the mutation of the light-dependent expressed sigma factor litS abolished the transcription in cryB2, phrB2 operon and cfa operon, indicating that LitR and LitS play a central role in the light-inducible transcription. Gel-shift assay showed that a recombinant protein LitR specifically binds to the promoter region of litR and folE2 operon, and its ability was lowered by UV-A illumination. LitR absorbs maximally near 340 nm, and exhibited a photocyclic response and light-dependent subunit dissociation. Furthermore, the litR mutant produced a 20-fold high intracellular folate compared to the wild-type strain. Overall, the line of evidence suggest that Class III LitR light-dependently regulates the transcription of litR itself and folE2-litS operon, leading the production of folate, and then expressed RNA polymerase-LitS directs the transcription for phrB2 operon and cfa operon. Light-dependent characteristics suggests that Class III LitR in complex with a UV-A absorbing molecule has a novel light-sensing mechanism.
 
Overall design Chromosomal RNA maps of wild-type cells cultured under the light conditions were compared with those of wild-type cells cultured under the dark conditions
 
Contributor(s) Ueda K, Takano H
Citation(s) 30249707
Submission date Mar 28, 2018
Last update date Oct 11, 2018
Contact name Hideaki Takano
E-mail(s) takano.hideaki@nihon-u.ac.jp
Phone 81466843936
Organization name Nihon University
Department College of Bioresource Sciences
Street address 1866, Kameino
City Fujisawa
State/province KANAGAWA
ZIP/Postal code 2520880
Country Japan
 
Platforms (1)
GPL24783 NimbleGen-Burkholderia multivorans ATCC 17616 385K 4-plex custom array [110225_RDKK301_Bmul_expr]
Samples (4)
GSM3070838 bmj14D
GSM3070839 bmj21D
GSM3070840 bmj14L
Relations
BioProject PRJNA447436

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112472_RAW.tar 10.8 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table
Processed data provided as supplementary file

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