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Status |
Public on Oct 09, 2018 |
Title |
bmj21D |
Sample type |
RNA |
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Source name |
cDNA prepared from dark culture of B. multivorans in middle stationary phase
|
Organism |
Burkholderia multivorans |
Characteristics |
strain: ATCC 17616 growth phase: middle stationary light status: dark culture
|
Growth protocol |
B. multivorans ATCC 17616 strain was grown in LB at 28°C at 135 strokes/min for 14 or 21 hrs under dark and light conditions.
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were treated with RNA Protect Bacteria reagent (Qiagen, CA, USA). Total RNA was extracted using RNeasy Midi kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
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Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
Description |
This sample is of wild-type Burkholderia multivorans cultured under the dark condition for 21 h.
|
Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
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Submission date |
Mar 28, 2018 |
Last update date |
Oct 09, 2018 |
Contact name |
Hideaki Takano |
E-mail(s) |
takano.hideaki@nihon-u.ac.jp
|
Phone |
81466843936
|
Organization name |
Nihon University
|
Department |
College of Bioresource Sciences
|
Street address |
1866, Kameino
|
City |
Fujisawa |
State/province |
KANAGAWA |
ZIP/Postal code |
2520880 |
Country |
Japan |
|
|
Platform ID |
GPL24783 |
Series (1) |
GSE112472 |
Role and function of Class III LitR, a photosensor homolog from Burkholderia multivorans ATCC 17616 in the regulation of folate production. |
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