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| Status |
Public on Apr 06, 2018 |
| Title |
Streptococcus suis 05ZY: wt vs SSU05_0930 knockout strain |
| Organism |
Streptococcus suis |
| Experiment type |
Expression profiling by array
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| Summary |
Two large-scale outbreaks of streptococcal toxic shock-like syndrome (STSLS) have revealed Streptococcus suis 2 (SS2) to be a severe, evolving pathogen in humans. We investigated the mechanism by which SS2 causes STSLS. The transcriptional regulator TstS up-regulated during experimental infection. Compared with the wild type 05ZY strain, the tstS deletion mutant (∆tstS) elicited reduced cytokine secretion in macrophages. In mice, tstS deletion decreased virulence, bacterial load, and cytokine production. Moreover, TstS expression in P1/7 strain led to induction of STSLS in the infected mice. This is noteworthy because although virulent, P1/7 does not normally induce STSLS. Through microarray-based comparative transcriptomics analysis, we found that TstS regulates multiple metabolism related genes and several virulence-related genes associated with immune evasion.
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| Overall design |
Streptococcus suis 05ZY (wt) and its SSU05_0930 knockout strain were harvested at the logarithmic phase of growth. Four independent experiments were performed at each time
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| Contributor(s) |
Xu Z, Chen B, Zhang Q, Liu L, Yan S, Yu J, Sun X, Jin M |
| Citation(s) |
29973920 |
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| Submission date |
Apr 05, 2018 |
| Last update date |
Jul 07, 2018 |
| Contact name |
Zhongmin Xu |
| E-mail(s) |
xzmjack@126.com
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| Phone |
+86 15827590798
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| Organization name |
Huazhong Agricultural University
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| Street address |
No.1,Shizishan Street
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| City |
Wuhan |
| State/province |
Hubei Province |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL20080 |
Agilent-022267 Streptococcus suis |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA448941 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112779_RAW.tar |
5.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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