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Series GSE112889 Query DataSets for GSE112889
Status Public on May 17, 2018
Title ZINC-FINGER interactions mediate transcriptional regulation of hypocotyl growth in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Integration of environmental signals and interactions among photoreceptors and transcriptional regulators is key in shaping plant development. TANDEM ZINC-FINGER PLUS3 (TZP) is an integrator of light and photoperiodic signaling that promotes flowering in Arabidopsis thaliana. Here we elucidate the molecular role of TZP as a positive regulator of hypocotyl elongation. We identify an interacting partner for TZP, the transcription factor ZINC-FINGER HOMEODOMAIN 10 (ZFHD10), and characterize its function in coregulating the expression of blue-light dependent transcriptional regulators and growth-promoting genes. By employing a genome-wide approach, we reveal that ZFHD10 and TZP coassociate with promoter targets enriched in light-regulated elements. Furthermore, using a targeted approach, we show that ZFHD10 recruits TZP to the promoters of key coregulated genes. Our findings not only unveil the mechanism of TZP action in promoting hypocotyl elongation at the transcriptional level but also assign a function to an uncharacterized member of the ZFHD transcription factor family in promoting plant growth.
Overall design We performed whole-genome chromatin immunoprecipitation (ChIP-seq) analysis on 7-day-old Arabidopsis thaliana Columbia wild-type (Col-0), OX TZP and OX ZFHD10 seedlings exposed to continuous low blue-light (LBL).
Contributor(s) Perrella G, Nastase AM, Herzyk P, Kaiserli E
Citation(s) 29686058
Submission date Apr 09, 2018
Last update date Sep 20, 2018
Contact name Eirini Kaiserli
Organization name University of Glasgow
Department Institute of Molecular, Cell & Systems Biology
Street address University Avenue
City Glasgow
ZIP/Postal code G12 8QQ
Country United Kingdom
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (3)
GSM3092813 Col-0 GFP low blue light _ChIPseq
GSM3092814 OX TZP GFP low blue light _ChIPseq
GSM3092815 OX ZFHD10 GFP low blue light _ChIPseq
BioProject PRJNA449688
SRA SRP139567

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Supplementary file Size Download File type/resource
GSE112889_RAW.tar 34.2 Mb (http)(custom) TAR (of BED, BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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