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| Status |
Public on May 17, 2018 |
| Title |
ZINC-FINGER interactions mediate transcriptional regulation of hypocotyl growth in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Integration of environmental signals and interactions among photoreceptors and transcriptional regulators is key in shaping plant development. TANDEM ZINC-FINGER PLUS3 (TZP) is an integrator of light and photoperiodic signaling that promotes flowering in Arabidopsis thaliana. Here we elucidate the molecular role of TZP as a positive regulator of hypocotyl elongation. We identify an interacting partner for TZP, the transcription factor ZINC-FINGER HOMEODOMAIN 10 (ZFHD10), and characterize its function in coregulating the expression of blue-light dependent transcriptional regulators and growth-promoting genes. By employing a genome-wide approach, we reveal that ZFHD10 and TZP coassociate with promoter targets enriched in light-regulated elements. Furthermore, using a targeted approach, we show that ZFHD10 recruits TZP to the promoters of key coregulated genes. Our findings not only unveil the mechanism of TZP action in promoting hypocotyl elongation at the transcriptional level but also assign a function to an uncharacterized member of the ZFHD transcription factor family in promoting plant growth.
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| Overall design |
We performed whole-genome chromatin immunoprecipitation (ChIP-seq) analysis on 7-day-old Arabidopsis thaliana Columbia wild-type (Col-0), OX TZP and OX ZFHD10 seedlings exposed to continuous low blue-light (LBL).
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| Contributor(s) |
Perrella G, Nastase AM, Herzyk P, Kaiserli E |
| Citation(s) |
29686058 |
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| Submission date |
Apr 09, 2018 |
| Last update date |
Sep 20, 2018 |
| Contact name |
Eirini Kaiserli |
| E-mail(s) |
eirini.kaiserli@glasgow.ac.uk
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| Organization name |
University of Glasgow
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| Department |
Institute of Molecular, Cell & Systems Biology
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| Street address |
University Avenue
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| City |
Glasgow |
| ZIP/Postal code |
G12 8QQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA449688 |
| SRA |
SRP139567 |
