NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE11311 Query DataSets for GSE11311
Status Public on Feb 11, 2009
Title Drosophila Sex Hierarchy Regulated Gene Expression in 48 hour APF Pupae
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. In somatic tissues at 48 hour After Puparium Formation (APF), 173 sex-biased transcripts that likely function downstream of the doublesex (dsx) branch of the sex determination hierarchy were identified. The mode of regulation of the sex-specific isoforms of DSX (DSX-F and DSX-M) was examined. It was determined that for most downstream targets, DSX-F and DSX-M regulate gene expression in the same manner, but that one isoform acts as a more potent regulator.
Keywords: wild type; genetic modification
 
Overall design All microarrays were dual channel with direct comparisons of male versus female or wild type versus mutant. All samples consist of whole body pupae collected at 48 hour After Puparium Formation (APF). For each experiment, four biological replicates were analyzed in a dye-swap design.
 
Contributor(s) Lebo MS, Sanders LE, Sun F, Arbeitman MN
Citation(s) 19216785
Submission date Apr 30, 2008
Last update date Mar 19, 2012
Contact name Matthew S Lebo
Organization name University of Southern California
Department Molecular and Copmutational Biology
Lab Arbeitman
Street address 1040 Child's Way
City Los Angeles
State/province CA
ZIP/Postal code 90254
Country USA
 
Platforms (1)
GPL6799 INDAC Drosophila melanogaster 15K v5.0
Samples (28)
GSM285605 Canton S 48 hr APF pupae - whole body - replicate 1
GSM285606 Canton S 48 hr APF pupae - whole body - replicate 2
GSM285643 Canton S 48 hr APF pupae - whole body - replicate 3
This SubSeries is part of SuperSeries:
GSE11316 Whole genome microarray analysis of the Drosophila morphogenetic process
Relations
BioProject PRJNA109201

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11311_RAW.tar 42.4 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap